Summary
Strains of Escherichia coli K12 were isolated in which the lac structural genes were fused to the promoter for the aminopeptidasee N structural gene (pepN). Although this enzyme is constitutively produced, the differential rate of synthesis is increased about 4-fold upon phosphate starvation. The pepN-lac fusions were shown to respond to phosphate specific regulatory signals. A plaque forming lambda transducing phage bearing the pepN-lac fusion was isolated. This phage was used to prove genetically the fusion of lac genes to the promoter for the aminopeptidase. These results demonstrate a control at the transcriptional level of aminopeptidase synthesis.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bermann ML, Beckwith J (1979) Use of gene fusions to isolate promoter mutants in the transfer RNA gene tyrT of Escherichia coli. J Mol Biol 130:303–315
Buxton RS (1979) Fusion of the lac genes to the proximal promoters of the deo operon of Escherichia coli K12. J Gen Microbiol 112:241–250
Casadaban MJ (1976) Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu. J Mol Biol 104:541–555
Debarbouille M, Schwartz M (1980) Mutants which make more mal T product, the activation of the maltose regular in Escherichia coli. Mol Gen Genet 178:589–595
Chippaux M, Bonnefoy-Orth V, Ratouchniak J, Pascal MC (1981) Operon fusions in the nitrate reductase operon and study of the control gene nirR in Escherichia coli. Mol Gen Genet 182:477–479
Eckardt T (1977) Use of argA-lac fusions to generate lambda aro-lac bacteriophages and to determine the direction of argA transcription in Escherichia coli. J Bacteriol 132:60–66
Feutrier J, Lepelletier M, Pascal MC, Chippaux M (1982) Tn10 insertions directed in the pyrD-serC region and improved mapping of pepN in Escherichia coli K12. Mol Gen Genet (in press)
Hall MN, Silhavy TJ (1979) Transcriptional regulation of Escherichia coli K12 major outer membrane protein 1 G. J Bacteriol 140:342–350
Hopkins JD (1974) A new class of promoter mutations in the lactose operon of Escherichia coli. J Mol Biol 87:715–724
Josephsen J, Hammer-Jespersen K (1981) Fusion of the lac genes to the promoter for the cytidine Deaminase gene of Escherichia coli K12. Mol Gen Genet 182:154–158
Lazdunski A, Murgier M, Lazdunski C (1975) Evidence for an aminopeptidase localized near the cell surface of Escherichia coli: regulation of synthesis by inorganic phosphate. Eur J Biochem 60:349–355
Murgier M, Pelissier C, Lazdunski A (1977) Aminopeptidase N from Escherichia coli unusual interactions with the cell surface. Eur J Biochem 74:425–433
Miller GG, Mackinnon K (1974) Peptidase mutants of Salmonella typhimurium. J Bacteriol 120:355–363
Miller JH (1972) Experiments in molecular genetics. New York, Cold Spring Harbor Laboratory
Saint-Girons I (1978) New regulatory mutants affecting the expression of the threonine operon in Escherichia coli K12. Mol Gen Genet 162:95–100
Shimada K, Weisberg R, Gottesman ME (1973) Prophage lambda at unusual chromosome locations: mutations induced by bacteriophage lambda in Escherichia coli K12. J Mol Biol 80:297–314
Toriani A (1960) Influence of inorganic phosphate in the formation of phosphatase by Escherichia coli. Biochim Biophys Acta 38:460–479
Author information
Authors and Affiliations
Additional information
Communicated by H. Böhme
Rights and permissions
About this article
Cite this article
Murgier, M., Gharbi, S. Fusion of the lac genes to the promoter for the aminopeptidase N gene of Escherichia coli . Molec Gen Genet 187, 316–319 (1982). https://doi.org/10.1007/BF00331136
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00331136