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Selection in Bacillus subtilis giving rise to strains with mutational alterations in a variety of ribosomal proteins

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Summary

To facilitate mapping of ribosomal protein genes in Bacillus subtilis, a selection was devised which gave rise to strains with alterations in any one of a variety or ribosomal proteins. Alterations in eighteen ribosomal proteins were identified when eighty mutants were analysed. In addition, one strain showed a major assembly defect in the large ribosomal subunit resulting in the presence of a particle sedimenting at about 40S. Eighteen large subunit proteins were present on this particle in normal amounts, while twelve proteins were much reduced in amount or undetectible.

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Authors and Affiliations

  1. Abt. Wittmann, Max-Planck-Institut für Molekulare Genetik, Ihnestraße 63-73, D-1000, Berlin 33, (Dahlem)

    Eric R. Dabbs

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  1. Eric R. Dabbs
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Communicated by A. Böck

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Dabbs, E.R. Selection in Bacillus subtilis giving rise to strains with mutational alterations in a variety of ribosomal proteins. Molec Gen Genet 187, 297–301 (1982). https://doi.org/10.1007/BF00331132

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  • DOI: https://doi.org/10.1007/BF00331132

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