Abstract
The regularly occurring secondary constrictions on metaphase chromosomes of mammalian cells prove to be nucleolus organizers as expected. The expression of nucleolus organizers as secondary constrictions, however, varies from cell to cell and from tissue to tissue, including cultivation in vitro. Electron micrographs of the organizer region show that the nucleolus organizer at metaphase is not a constriction. The width of the organizer area is the same as the condensed chromosomal arms; but the filaments, which are the major components of this region, show a diameter of 50–70 Å. The condensed chromosome arms consist of filaments 150–200 Å in diameter. In some mammalian species, structures similar to the nucleolus organizer are located at the end of chromosomes. These may be terminal nucleolus organizers.
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Arrighi, F. E.: Nucleolar RNA synthetic activity in Chinese hamster cells in vitro and the effects of actinomycin D and nogalamycin. J. Cell. Physiol. 69, 45–52 (1967).
Attardi, G., P. -C. Huang, and S. Kabat: Recognition of ribosomal RNA sites in DNA. II. The HeLa cell system. Proc. nat. Acad. Sci. (Wash.) 54, 185–192 (1965).
Bayreuther, K.: Der Chromosomenbestand des Ehrlich Ascites Tumors der Maus. Z. Naturforsch. 7b, 554–557 (1952).
Biedler, J. L., L. J. Old, and D. A. Clarke: Chromosomal lesion associated with carcinogen induced tumor in mice. Nature (Lond.) 192, 286–288 (1961).
Brinkley, B. R., P. Murphy, and L. C. Richardson: Procedure for embedding in situ selected cells cultured in vitro. J. Cell Biol. 35, 279–283 (1967).
—, and E. Stubblefield: Fine structure of the kinetochore of Chinese hamster cells in vitro. Chromosoma (Berl.) 19, 28–43 (1966).
Brown, D. D., and J. B. Gurdon: Absence of ribosomal RNA synthesis in the anucleolate mutant of Xenopus laevis. Proc. nat. Acad. Sci. (Wash.) 51, 139–146 (1964).
Callan, H. G.: Chromosomes and nucleoli of the axolotl, Ambystoma mexicanum. J. Cell Sci. 1, 85–108 (1966).
Das, N. K., and M. Alfert: Nucleolar RNA synthesis during mitotic and meiotic prophase. Nat. Cancer Inst. Monogr. 23, 337–351 (1966).
Gabrusewycz-Garcia, N., and R. G. Kleinfeld: A study of the nucleolar material in Sciara coprophila. J. Cell Biol. 29, 347–359 (1966).
Hayman, D. L., and P. G. Martin: An autoradiographic study of DNA synthesis in the sex chromosomes of two Marsupials with an XX/XY1Y2 sex chromosome mechanism. Cytogenetics 4, 209–218 (1965).
Heitz, E.: Die Ursache der gesetzmäßigen Zahl, Lage, Form und Größe pflanzlicher Nukleolen. Planta (Berl.) 12, 774–844 (1931).
Heneen, W. K., and W. W. Nichols: Persistence of nucleoli in short term and long term cell cultures and in direct bone marrow preparations in mammalian materials. J. Cell Biol. 31, 543–561 (1966).
Hsu, T. C., F. E. Arrighi, R. R. Klevecz, and B. R. Brinkley: The nucleoli in mitotic divisions of mammalian cells in vitro. J. Cell Biol. 26, 539–553 (1965).
—, and C. E. Somers: Effect of 5 bromodeoxyuridine on mammalian chromosomes. Proc. nat. Acad. Sci. (Wash.) 47, 396–402 (1961).
Levan, A., T. C. Hsu, and H. F. Stich: The idiogram of the mouse. Hereditas (Lund) 48, 677–687 (1962).
Luft, J. H.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol. 9, 409–414 (1961).
McClintock, B.: The relation of a particular chromosomal element to the development of the nucleoli in Zea mays. Z. Zellforsch. 21, 294–328 (1934).
McConkey, E. H., and J. W. Hopkins: The relationship of the nucleolus to the synthesis of ribosomal RNA in HeLa cells. Proc. nat. Acad. Sci. (Wash.) 51, 1197–1204 (1964).
Miles, C. P., and F. O'Nell: Prominent secondary constrictions in a pseudodiploid human cell line. Cytogenetics 5, 321–334 (1966).
Millonig, G.: Advantages of a phosphate buffer for OsO4 solutions in fixation. J. appl. Phys. 32, 1637 (1961).
Perry, R. P.: Role of the nucleolus in ribonucleic acid metabolism and other cellular processes. Nat. Cancer Inst. Monogr. 14, 73–89 (1964).
Prescott, D. M., and M. A. Bender: Synthesis of RNA and protein during mitosis in mammalian tissue culture cells. Exp. Cell Res. 26, 260–268 (1962).
Report of the Nucleolus Nomenclature Comittee (H. Swift, Chmn): Nat. Cancer Inst. Monogr. 23, 573–574 (1966).
Ritossa, F. M., K. C. Atwood, D. L. Lindsley, and S. Spiegelman: On the chromosomal distribution of DNA complementary to ribosomal and soluble RNA. J. Nat. Cancer Inst. Monogr. 23, 449–472 (1966).
Sharman, G. B., and H. N. Barber: Multiple sex chromosomes in the Marsupial Potorous. Heredity 6, 345–355 (1952).
Shaw, M. W., and R. S. Krooth: The chromosomes of the Tasmanian rat kangaroo (Potorous tridactylis apicalis). Cytogenetics 3, 19–33 (1964).
Stich, H. F., T. C. Hsu, and F. Rapp: Viruses and mammalian chromosomes. I. Localization of chromosome aberrations after infection with Herpes simplex virus. Virology 22, 439–445 (1964).
Taylor, J. H.: Nucleic acid synthesis in relation to the cell division cycle. Ann. N. Y. Acad. Sci. 90, 409–421 (1960).
Utakoji, T.: Chronology of nucleic acid synthesis in meiosis of the male Chinese hamster. Exp. Cell Res. 42, 585–596 (1966).
—, and T. C. Hsu: DNA replication patterns in somatic and germ line cells of the male Chinese hamster. Cytogenetics 4, 295–315 (1965).
Vatter, A. E., and J. Zambernand: A comparative study of water soluble plastic and alcohol dehydrated tissues. Abstract of Electron Microscopy Society of America, San Francisco, Calif. T-12 (1966).
Walen, K. H., and S. W. Brown: Chromosomes in a Marsupial (Potorous tridactylis) tissue culture. Nature (Lond.) 194, 406 (1962).
Wallace, H., and M. L. Birnstiel: Ribosomal cistrons and the nucleolar organizer. Biochim. biophys. Acta (Amst.) 114, 296–310 (1966).
Wolman, S. R., K. Hirschhorn, and G. J. Todaso: Early chromosomal changes in SV40-infected human fibroblast cultures. Cytogenetics 3, 45–46 (1964).
Yerganian, G., R. Kato, M. L. Leonard, H. J. Gagnon, and M. S. Grodzins: Sex chromosomes in malignancy, transplantability of growths, and aberrant sex determination. In: Cell physiology of neoplasia, p. 49–96. Austin: University of Texas Press (1960).
—, and S. Papoyan: Isomorphic sex chromosomes, autosomal heteromorphism, and telomeric associations in the grey hamster of Armenia, Cricetulus migratorius Pall. Hereditas (Lund) 52, 307–319 (1964).
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Supported in part by Research Grants DRG-269 from Damon Runyon Memorial Fund for Cancer Research, E-286 from American Cancer Society, and HD-2590 from National Institutes of Health.
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Hsu, T.C., Brinkley, B.R. & Arrighi, F.E. The structure and behavior of the nucleolus organizers in mammalian cells. Chromosoma 23, 137–153 (1967). https://doi.org/10.1007/BF00331109
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DOI: https://doi.org/10.1007/BF00331109