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Ultrastructural localization of monoamines in the epidermis of Lumbricus terrestris (L.)

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Summary

Receptor cells in the epithelium and the basiepithelial nerve net of the prostomium of Lumbricus terrestris were investigated with electron microscope with special regard to the presence of monoamines. The receptor cells are found in groups of about 40 intermingled with supportive cells. After pretreatment with α-methyl-noradrenaline and fixation with potassium permanganate a few receptor cells in each group and some nerve fibres in the basiepithelial nerve net contain small granular vesicles (about400 Å) characteristic for monoaminergic neurons. The distribution and relative number of these receptor cells and nerve fibres coincide well with previous reports on fluorescent receptor cells and varicose fibres. That the monoamine-storing small granular vesicles not are visualized until pretreatment with α-methyl-noradrenaline is in accordance with recent microspectrofluorometric analysis, which shows that dopamine is the only primary monoamine present in the epithelium.

In the epithelium there are occasional receptor cells and nerve fibres containing large vesicles (1000–1800 Å) which resemble the neurosecretory vesicles in the central nervous system. Photoreceptor cells having an intracellular cavity with microvilli and cilia have infrequently been observed at the base of the epithelium.

No synapses on the mucous cells have been noticed. Nor have any synaptic specializations been observed in the basiepithelial nerve net. The morphological conditions necessary for the existence of possible axo-axonal synapses are briefly discussed.

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  1. Zoological Institute, Lund, Sweden

    Harry E. Myhrberg

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  1. Harry E. Myhrberg
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This work was supported by grants from the Helge Ax: son Johnson Foundation and the Magn. Bergvall Foundation.

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Myhrberg, H.E. Ultrastructural localization of monoamines in the epidermis of Lumbricus terrestris (L.). Z. Zellforsch. 117, 139–154 (1971). https://doi.org/10.1007/BF00331107

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