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Cloning of a Streptomyces gene for an O-methyltransferase involved in antibiotic biosynthesis

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Summary

The red pigmented antibiotic of Streptomyces coelicolor A3(2) is chemically very similar to the Serratia marcescens pigment, prodigiosin. We have demonstrated by co-synthesis experiments between non-producing mutants of both species that their biosynthetic pathways are similar, and have discovered identities between specific mutants of each organism. Molecular cloning techniques have been employed in order to isolate Streptomyces chromosomal DNA segments which “complement” a mutant defective in the penultimate step of the red biosynthetic pathway: an O-methyltransferase enzyme. In one case, the lesion appears to be repaired by integrative recombination into the chromosome; another case may represent expression from the autonomously replicating recombinant plasmid.

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References

  • Bibb MJ, Schottel JL, Cohen SN (1980) A DNA cloning system for interspecies gene transfer in antibiotic-producing Streptomyces. Nature 284:526–531

    Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Google Scholar 

  • Chater KF, Hopwood DA, Kieser T, Thompson CJ (1982) Gene cloning in Streptomyces. Curr Topics Microbiol and Immunol 96:69–95

    Google Scholar 

  • Gerber NN (1975) Prodigiosin-like pigments. CRC Critical Rev Microbiol 3:469–485

    Google Scholar 

  • Gerber NN, Lechevalier MP (1976) Prodiginine (prodigiosin-like) pigments from Streptomyces and other aerobic actinomycetes. Can J Microbiol 22:658–667

    Google Scholar 

  • Grosveld FG, Dahl H-HM, deBoer E, Flavell RA (1981) Isolation of β-globin-related genes from a human cosmid library. Gene 13:227–237

    Google Scholar 

  • Hopwood DA (1981) Future possibilities for the discovery of new antibiotics by genetic engineering. In: Salton MRJ, Shockman GD (eds) β-lactam antibiotics. Academic Press, New York, pp 585–598

    Google Scholar 

  • Hopwood DA, Chater KF (1980) Fresh approaches to antibiotic production. Phil Trans Roy Soc B 290:313–328

    Google Scholar 

  • Hopwood DA, Chater KF (1982) Cloning in Streptomyces: systems and strategies. In: Setlow JK, Hollaender A (eds) Genetic engineering, vol 4. Plenum Press, New York, pp 119–145

    Google Scholar 

  • Katz E, Thompson CJ, Hopwood DA (1983) Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans (Submitted)

  • Kieser T, Hopwood DA, Wright HM, Thompson CJ (1982) pIJ101, a multi-copy broad host-range Streptomyces plasmid: functional analysis and development of DNA cloning vectors. Mol Gen Genet 185:223–238

    Google Scholar 

  • Rudd BAM, Hopwood DA (1979) Genetics of actinorhodin biosynthesis by Streptomyces coelicolor A3(2). J Gen Microbiol 114:35–43

    Google Scholar 

  • Rudd BAM, Hopwood DA (1980) A pigmented mycelial antibiotic in Streptomyces coelicolor: control by a chromosomal gene cluster. J Gen Microbiol 119:333–340

    Google Scholar 

  • Seno ET, Baltz RH (1981) Properties of S-adenosyl-L-methionine: macrocin O-methyltransferase in extracts of Streptomyces fradiae strains which produce normal or elevated levels of tylosin and in mutants blocked in specific O-methylations. Antimicrob Agents Chemother 20:370–377

    Google Scholar 

  • Seno ET, Bruton CJ, Chater KF (1983) The glycerol utilization operon of Streptomyces coelicolor — genetic mapping of gyl mutations and analysis of clones gyl DNA. Mol Gen Genet (submitted)

  • Suarez JE, Chater KF (1980) DNA cloning in Streptomyces: a bifunctional replicon comprising pBR322 inserted into a Streptomyces phage. Nature 286:527–529

    Google Scholar 

  • Thompson CJ, Ward JM, Hopwood DA (1980) DNA cloning in Streptomyces: resistance genes from antibiotic-producing species. Nature 286:525–527

    Google Scholar 

  • Thompson CJ, Ward JM, Hopwood DA (1982) Cloning of antibiotic resistance and nutritional genes in streptomycetes. J Bacteriol 151:668–677

    Google Scholar 

  • Wasserman HH, Shaw CK, Sykes RJ, Cushey RJ (1974) The biosynthesis of metacycloprodigiosin and undecylprodigiosin. Tetra Letters 33:2787–2790

    Google Scholar 

  • Williams RP, Qadri SMH (1980) The pigment of Serratia. In: von Graevenitz A, Rubin SJ (eds) The genus Serratia. CRC Press, Boca Raton, pp 31–75

    Google Scholar 

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Authors and Affiliations

  1. John Innes Institute, Colney Lane, NR4 7UH, Norwich, UK

    Jerald S. Feitelson & David A. Hopwood

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  1. Jerald S. Feitelson
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  2. David A. Hopwood
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Communicated by D. Sherratt

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Feitelson, J.S., Hopwood, D.A. Cloning of a Streptomyces gene for an O-methyltransferase involved in antibiotic biosynthesis. Mol Gen Genet 190, 394–398 (1983). https://doi.org/10.1007/BF00331065

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  • DOI: https://doi.org/10.1007/BF00331065

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