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Chromosome banding and genome compartmentalization in fishes

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Abstract

We have investigated the correlation between chromosome banding and compositional compartmentalization of the genome in three selected fish species, Anguilla anguilla (Anguilliformes), Epinephelus guttatus (Perciformes), and Labeo bicolor (Cypriniformes). These species were selected because they provided a spectrum of such compartmentalizations. Indeed, the band profiles in CsCl density gradients of the corresponding DNAs are characterized by a high compositional heterogeneity and a strong asymmetry for A. anguilla and by a very low compositional heterogeneity and a very weak asymmetry for E. guttatus. L. bicolor DNA shows an intermediate situation. — Chromosomes were studied using three consecutive stainings of the same mitoses. The intensity and reciprocity of the patterns obtained after Q- and R-banding were relatively strong for A. anguilla, whereas essentially no banding was found for E. guttatus. L. bicolor exhibited a chromosome banding of intermediate intensity and reciprocity. There is therefore a parallelism between chromosome banding and compositional compartmentalization of the genome. This finding provides new, independent support for previous indications of a correlation between these two properties.

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Author notes

  1. Leandro Medrano

    Present address: Department of Cell Biology, Universidad Autonoma de Barcelona, Bellaterra, Barcelona, Spain

Authors and Affiliations

  1. Laboratoire de Génétique Moléculaire, Institut Jacques Monod, 2 Place Jussieu, F-75005, Paris, France

    Leandro Medrano, Giacomo Bernardi & Giorgio Bernardi

  2. Unité CNRS “Structure et Mutagénèse Chromosomiques”, Institut Curie, 26 Rue d'Ulm, F-75005, Paris, France

    Jérome Couturier & Bernard Dutrillaux

Authors
  1. Leandro Medrano
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  2. Giacomo Bernardi
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  3. Jérome Couturier
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  4. Bernard Dutrillaux
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  5. Giorgio Bernardi
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Medrano, L., Bernardi, G., Couturier, J. et al. Chromosome banding and genome compartmentalization in fishes. Chromosoma 96, 178–183 (1988). https://doi.org/10.1007/BF00331050

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  • DOI: https://doi.org/10.1007/BF00331050

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