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Transformation of yeast and Podospora: innocuity of senescence-specific DNAs

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Summary

Two senscence-specific DNAs (sen-DNAs α and β) were tested for their ability to drive autonomous replication in yeast and Podospora. The β but not the α sequence has autoreplicative (ARS) properties in yeast; the ARS sequences of β are not included in the region common to all the β sen-DNAs. Neither the α nor the β sequences can confer autoreplicative properties in Podospora. These sequences inserted into a hybrid vector carrying the suppressor tRNA, su4-1, do not change the mode of transformation of a suppressible leu-1-1 strain of Podospora: the transformation is by integration whether or not the plasmid carries a sen-DNA sequence. The su4-1 gene integrates at its homologous site in a minority of cases. It is possible to reisolate free plasmids at a low frequency from some transformants. The presence of a sen-DNA on the transforming vector has no effect upon the longevity of the transformants.

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Authors and Affiliations

  1. Centre de Génétique Moléculaire, Centre National de la Recherche Scientifique, F-91190, Gif-sur-Yvette, France

    Annie Sainsard-Chanet & Odile Begel

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  1. Annie Sainsard-Chanet
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  2. Odile Begel
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Communicated by W. Gajewski

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Sainsard-Chanet, A., Begel, O. Transformation of yeast and Podospora: innocuity of senescence-specific DNAs. Molec Gen Genet 204, 443–451 (1986). https://doi.org/10.1007/BF00331022

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  • DOI: https://doi.org/10.1007/BF00331022

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