Summary
Phage Mu-1 mediated transposition has been used to map genes coding for ribosomal proteins and elongation factor G inside transcriptional units. The data indicate that 1) the strA and fusA genes belong to the same operon, 2) the spcA and strA genes are expressed independently, 3) the spcA gene is located in a different transcriptional unit to that of the eryA and eryB genes.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bachmann, J.-B., Low, K.B., Taylor, A.-L.: Recalibrated linkage map of Escherichia coli K12. Bact. Rev. 40, 116–167 (1976)
Brown, M.-E., Apirion, D.: Mapping a cluster of ribosomal genes in Escherichia coli. Molec. gen. Genet. 133, 317–321 (1974)
Cabezón, T., Delcuve, G., Herzog, A., Petre, J., Bollen, A.: Characterization of conditionally lethal mutations affecting ribosomal proteins in Escherichia coli. Biochem. Soc. Trans. 5, 958–959 (1977a)
Cabezón, T., Herzog, A., Petre, J., Yaguchi, M., Bollen, A.: Ribosomal assembly deficiency in an Escherichia coli thermosensitive mutant having an altered L24 ribosomal protein. J. molec. Biol. 116, 361–374 (1977b)
Faelen, M., Toussaint, A.: Bacteriophage Mu-1, a tool to transpose and to localized bacterial genes. J. molec. Biol. 104, 523–539 (1976)
Faelen, M., Toussaint, A., van Montagu, M., van den Elsacker, S., Engler, G., Schell, J.: In vivo genetic engeneering: Mumediated transposition of chromosomal DNA onto transmissible plasmids in: Insertion sequences, plasmids and episomes (Bukhari, A.I., Shapiro, J., Adhya, S., eds.), pp. 521–530. New York: Cold Spring Harbor Press 1977
Glansdorff, N.: Topography of cotransducible arginine mutations in E. coli K12. Genetics 51, 167–173 (1965)
Howe, M.M.: Prophage deletion mapping bacteriophage Mu-1. Virology 54, 93–101 (1973)
Jaskunas, S.R., Nomura, M., Davies, J.: Genetics of bacterial ribosomes. In: Ribosomes, pp. 333–368. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory 1974
Lennox, E.S.: Transduction of linked genetic characters of the host by bacteriophage P1. Virology 1, 190–206 (1955)
Low, B.: Formation of merodiploids in matings with a class of rec- recipient strains of E. coli K12. Proc. nat. Acad. Sci. (Wash.) 60, 160–167 (1968)
Miller, J.: Experiments in molecular biology. New York: Cold Spring Harbor press 1972
Nomura, M.: Organization of bacterial genes for ribosomal components: studies using novel approaches. Cell 9, 633–644 (1976)
Taylor, A.L.: Bacteriophage induced mutations in E. coli. Proc. nat. Acad. Sci. (Wash.) 50, 1043–1051 (1963)
Wittmann, H.G., Stüffler, G., Apirion, D., Rosen, L., Tanaka, K., Tamaki, M., Takata, R., Dekio, S., Otaka, E., Osawa, S.: Biochemical and genetic studies on two different types of erythromycin resistant mutants of E. coli with altered ribosomal proteins. Molec. gen. Genet. 127, 175–189 (1973)
Wittmann, H.G., Yaguchi, M., Piepersberg, W., Böck, A.: Direction of Transcription of two ribosomal protein genes in Escherichia coli. J. molec. Biol. 98, 827–830 (1975)
Author information
Authors and Affiliations
Additional information
Communicated by H.G. Wittmann
Rights and permissions
About this article
Cite this article
Cabezón, T., Van Gijsegem, F., Toussaint, A. et al. Phage Mu-1 mediated transposition: A tool to study the organization of ribosomal protein genes in Escherichia coli . Molec. gen. Genet. 161, 291–296 (1978). https://doi.org/10.1007/BF00331003
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00331003