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Mutations in the tryptophanyl-transfer ribonucleic acid ligase of E. coli causing temperature-sensitivity for growth

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Summary

Strains of Escherichia coli K-12 with altered tryptophanyl-tRNA ligases, conferring temperature-sensitivity for growth, have been isolated as spontaneous one-step mutants. The mutated enzymes differ markedly in activity from the wildtype, even at the permissive temperature. When assayed at the non-permissive temperature, the mutant enzymes are completely inactive. In one of the mutant strains, growth can be completely inhibited by addition of L-phenylalanine or L-tyrosine to the medium.

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Authors and Affiliations

  1. The Wallenberg Laboratory, Molecular Biology Institute, University of Uppsala, S-752 37, Uppsala, Sweden

    Kaj Bohman & Leif A. Isaksson

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  1. Kaj Bohman
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  2. Leif A. Isaksson
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Communicated by E. Bautz

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Bohman, K., Isaksson, L.A. Mutations in the tryptophanyl-transfer ribonucleic acid ligase of E. coli causing temperature-sensitivity for growth. Molec. gen. Genet. 161, 285–289 (1978). https://doi.org/10.1007/BF00331002

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  • DOI: https://doi.org/10.1007/BF00331002

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