Summary
Crude extracts from λ-lysogens treated with mitomycin C were prepared, and immunity repressor levels in the extracts were assayed by the binding activity specific to λ DNA immunity region. It has been shown that while the repressor levels in the extracts from C600(λ+) are reduced after mitomycin C treatment, the levels in C600recA(λ+) and C600 C72(λ+) which have defects in lifting the immunity are not affected by the treatment. The repressor levels in the extracts prepared from C600 T44(λ+) after temperature shift up, whose prophage is inducible at high temperature, are also reduced. From the study of chloramphenicol effect, it was indicated that de novo protein synthesis is required for the inactivation of the repressor in C600(λ+) by mitomycin C, but not in T44(λ+) by high temperature.
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References
Echols, H., Green, L.: Establishment and maintenance of repression by bacteriophage lambda: The role of the CI, CII, and CIII proteins. Proc. nat. Acad. Sci. (Wash.) 68, 2190–2194 (1971)
Kirby, E. P., Jacob, F., Goldthwait, D. A.: Prophage induction and filament formation in a mutant strain of Escherichia coli. Proc. nat. Acad. Sci. (Wash.) 58, 1903–1910 (1967)
Noack, D., Klaus, S.: Inactivation kinetics of Lambda phage repressors in a mutant of E. coli temperature sensitive in DNA replication. Molec. gen. Genet. 115, 216–224 (1972)
Ptashne, M.: Specific binding of the λ phage repressor to λ DNA. Nature (Lond.) 214, 232–234 (1967)
Reichardt, L., Kaiser, A. D.: Control of repressor synthesis. Proc. nat. Acad. Sci (Wash.) 68, 2185–2189 (1971)
Ruff, W., Kirby, E. P., Goldthwait, D. A.: Cell division and prophage induction in Escherichia coli: Studies of nucleotide levels. J. Bact. 106, 994–1004 (1971)
Signer, E. R.: Recombination between coliphages λ and ϕ80. Virology 22, 650–651 (1964)
Sussman, R., Jacob, F.: Sur un système de répression thermosensible chez le bactériophage λ d'Escherichia coli. C. R. Acad. Sci. (Paris) 254, 1517–1519 (1962)
Tomizawa, J., Ogawa, T.: Effect of ultraviolet irradiation on bacteriophage lambda immunity. J. molec. Biol. 23, 247–263 (1967)
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Communicated by H. Ozeki
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Shinagawa, H., Itoh, T. Inactivation of DNA-binding activity of repressor in extracts of λ-lysogen treated with mitomycin C. Molec. gen. Genet. 126, 103–110 (1973). https://doi.org/10.1007/BF00330987
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DOI: https://doi.org/10.1007/BF00330987