Summary
We have isolated restriction fragments from a “shotgun” collection of Drosophila DNA which function as autonomously replicating sequences (ARS) in Saccharomyces cerevisiae and hybridize with telomeric regions of the 2L, 2R, 4, and X chromosomes. In an independently obtained set of D. melanogaster clones five fragments hybridize in situ with telomeres and a number of internal sites. Two of them also contain ARSs. A Drosophila mobile P-element also possesses ARS activity in yeast.
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Communicated by D.M. Goldfarb
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Danilevskaya, O.N., Kurenova, E.V., Leibovitch, B.A. et al. Telomeres and P-element of Drosophila melanogaster contain sequences that replicate autonomously in Saccharomyces cerevisiae . Mol Gen Genet 197, 342–344 (1984). https://doi.org/10.1007/BF00330983
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DOI: https://doi.org/10.1007/BF00330983