Summary
Streptomyces ambofaciens strain ATCC23877 contains the 11.1 kb plasmid pSAM2 stably integrated into its chromosome. This plasmidic sequence is able to loop out and to be transferred at high frequency to S. lividans where it is found simultaneously as both free and integrated plasmid. When a UV derivative of strain ATCC23877 (strain ATCC15154) is used, the resident copy of pSAM2 can be transferred to S. lividans, but only the integrated form is found in this strain. In both cases, the integration occurs at a unique chromosomal region through the same plasmidic integration site as that in strain ATCC23877. The resident copy of strain ATCC15154 can also be transferred at low frequency to S. ambofaciens DSM40697 (devoid of any pSAM2 sequence). In this case, as several copies of pSAM2 are integrated, the integration pattern is complicated. Integration of a complete pSAM2 sequence in this strain occurs in a region that hybridizes with the integration zones of S. lividans and of S. ambofaciens strain ATCC23877. Comparison of the cloned integration zone of S. lividans before and after the integration event showed that the restriction pattern of the resident pSAM2 in strain ATCC15154 is similar to that of the free form of pSAM2 found naturally in another UV derivative of strain ATCC23877 (strain JI3212).
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References
Bibb MJ, Freeman RF, Hopwood DA (1977) Physical and genetical characterization of a second sex factor, SCP2 for Streptomyces coelicolor A3(2). Mol Gen Genet 154: 155–166
Bibb MJ, Ward JM, Hopwood DA (1978) Transformation of plasmid DNA into Streptomyces at high frequency. Nature 274: 398–400
Bibb MJ, Ward JM, Kieser T, Cohen SN, Hopwood DA (1981) Excision of chromosomal DNA sequences from Streptomyces coelicolor forms a novel family of plasmids detectable in Streptomyces lividans. Mol Gen Genet 184: 230–240
Calendar R, Ljungquist E, Deho G, Usher DC, Goldstein R, Youderian P, Sironi G, Six EW (1981) Lysogenization by satellite phage P4. Virology 113: 20–38
Caruso M, Shapiro JA (1982) Interactions of Tn7 and temperate phage F116L of Pseudomonas aeruginosa. Mol Gen Genet 188: 292–298
Covarrubias L, Bolivar F (1982) Construction and characterization of new cloning vehicles VI. Plasmid pBR329, a new derivative of pBR328 lacking the 482-base-pair inverted duplication. Gene 17: 79–89
Ely B (1982) Transposition of Tn7 occurs at a single site on the Caulobacter crescentus chromosome. J Bacteriol 151: 1056–1058
Hanahan D (1983) Studies on transformation of Escherichia coli with plasmids J Mol Biol 166: 557–580
Hintermann D, Crameri R, Kieser T, Hütter R (1981) Restriction analysis of the Streptomyces glaucescens genome by agarose gel electrophoresis. Arch Microbiol 130: 218–222
Hohn B (1979) In vitro packaging of λ and cosmid DNA. Methods Enzymol 68: 299–309
Hohn B, Collins J (1980) A small cosmid for efficient cloning of large DNA fragments. Gene 11: 291–298
Hopwood DA, Wright HM (1973) Transfer of a plasmid between Streptomyces species. J Gen Microbiol 77: 187–195
Hopwood DA, Wright HM (1976) Interactions of the plasmid SCP1 with the chromosome of Streptomyces coelicolor A3(2). In McDonald KD (ed) Second International Symposium on the Genetics of Industrial Microorganisms. Academic Press, London, pp 607–619
Hopwood DA, Kieser T, Wright HM, Bibb MJ (1983) Plasmids, recombination and chromosome mapping in Streptomyces lividans 66. J Gen Microbiol 129: 2257–2269
Hopwood DA, Hintermann G, Kieser T, Wright HM (1984) Integrated DNA sequences in three Streptomycetes form related autonomous plasmids after transfer to Streptomyces lividans. Plasmid 11: 1–16
Hopwood DA, Kieser T, Lydiate DJ, Bibb MJ (1986) Streptomyces plasmids: Their biology and use as cloning vectors. In: Quenner JW, Day LE (eds) The bacteria, vol IX. Academic Press, Orlando, pp 159–229
Katz L, Brown DP, Tuan JS, Chiang SD (1988) Autonomous replication and site-specific integration in Streptomyces lividans of plasmid pSE101 that exists in the chromosome of Streptomyces erythreus. J Bacteriol (in press)
Kendall K, Cullum J (1986) Identification of a DNA sequence associated with plasmid integration in Streptomyces coelicolor A3(2). Mol Gen Genet 202: 79–88
Kieser T (1984) Factors affecting the isolation of ccc DNA from Streptomyces lividans and Escherichia coli. Plasmid 12: 19–36
Kinashi H, Shimaji M, Sakai A (1987) Giant linear plasmids in Streptomyces which code for antibiotic biosynthesis genes. Nature 328: 454–456
Leong JM, Nunes-Düby S, Lesser CF, Youderian P, Susskind MM, Landy A (1985) The ϕ80 and P22 attachment sites. J Biol Chem 260: 4468–4477
Lichtenstein C, Brenner S (1982) Unique insertion site of Tn7 in the E. coli chromosome Nature 297: 601–603
Lydiate DJ, Ikeda H, Hopwood DA (1986) A 2.6 kb DNA sequence of Streptomyces coelicolor A3(2) which functions as a transposable element. Mol Gen Genet 203 79–88
Madon J, Moretti P, Hütter R (1987) Site-specific integration and excision of pMEA100 in Nocardia mediterranei. Mol Gen Genet 209: 257–264
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Messing J (1983) New M13 vectors for cloning. Methods Enzymol 101: 20–78
Messing J, Crea R, Seeburg PH (1981) A system for shotgun DNA sequencing. Nuclei Acids Res 9: 309–321
Moretti P, Hintermann G, Hütter R (1985) Isolation and characterization of an extrachromosomal element from Nocardia mediterranei. Plasmid 14: 126–133
Murphy E, Phillips S, Edelman I, Novick RP (1981) Tn554: isolation and characterization of plasmid insertions. Plasmid 5: 292–305
Nash HA (1981) Integration and excision of bacteriophage λ: the mechanism of conservative site specific recombination. Annu Rev Genet 15: 143–167
Omer CA, Cohen SN (1984) Plasmid formation in Streptomyces: Excision and integration of the SLP1 replicon at a specific chromosomal site. Mol Gen Genet 196: 429–438
Omer CA, Cohen SN (1986) Structural analysis of plasmid and chromosomal loci involved in site-specific excision and integration of the SLP1 element of Streptomyces coelicolor. J Bacteriol 166: 999–1006
Pernodet JL, Simonet JM, Guerineau M (1984) Plasmids in different strains of Streptomyces ambofaciens: free and integrated form of plasmid pSAM2. Mol Gen Genet 198: 35–41
Pridham TG, Anderson P, Foley C, Lindenfelser LA, Hesseltine CW, Benetdict RC (1957) A selection of media for maintenance and taxonomic study of Streptomyces. Antibiotics Annual 1956–1957, pp 947–953
Rigby PWJ, Dieckmann M, Rhodes C, Berg P (1977) Labelling deoxyribonucleic acid to high specific activity in vitro by nick-translation with DNA polymerase I. J Mol Biol 113: 237–251
Simonet JM, Boccard F, Pernodet JL, Gagnat J, Guerineau M (1987) Excision and integration of a self-transmissible replicon of Streptomyces ambofaciens. Gene 59: 137–144
Smokvina T, Francou F, Luzzati M (1988) Genetic analysis in Streptomyces ambofaciens. J Gen Microbiol 134: 395–402
Thompson CJ, Ward JM, Hopwood DA (1982) Cloning of antibiotic resistance and nutritional genes in Streptomycetes. J Bacteriol 151: 668–677
Vivian A (1971) Genetic control of fertility in Streptomyces coelicolor A3(2): plasmid involvement in the interconversion of UF and IF strains. J Gen Microbiol 69: 353–364
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Communicated by W. Gajewski
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Boccard, F., Pernodet, JL., Friedmann, A. et al. Site-specific integration of plasmid pSAM2 in Streptomyces lividans and S. ambofaciens . Mol Gen Genet 212, 432–439 (1988). https://doi.org/10.1007/BF00330847
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DOI: https://doi.org/10.1007/BF00330847