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UV-mutable hybrids of Salmonella incorporating escherichia coli Region adjacent to Tryptophan operon

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Summary

Salmonella typhimurium recombinants have been constructed which have acquired UV-induced mutability. Such recombinants were obtained after transfer of plasmid F′126 from E. coli. UV-mutability was acquired by UV-stable Salmonella as a nonselective marker after selection of trp + marker in 2.5% of the cases. Possible deficiency in UV-stable Salmonella of the umuC gene is discussed.

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References

  • Andereeva IV, Aleshkin GI, Stepanova NF, Skavronskaya AG (1980) Influence of plasmids on UV-induction of prophages in S. typhimurium and E. coli (1980) In: Structure and functions of Plasmids. (ed. Domaradsky), Moscow, p 17–18

  • Bachmann BI, Low KB (1980) Linkage map of Escherichia coli K-12. Edition 6. Microb Rev 44:1–56

    Google Scholar 

  • Böhme H (1963) Mutagen stability of Proteus mirabilis against UV-and X-irradiation. Microb Genet Bull 19:5–12

    Google Scholar 

  • Diaz R, Barnsley P, Pritchard RH (1979) Location and characterisation of a new replication origin in the E. coli K-12 chromosome. Mol Gen Genet 175:151–157

    Google Scholar 

  • Dobson PP, Walker G (1980) Plasmid (pKM101)-mediated Weigle reactivation in Escherichia coli K-12 and Salmonella typhimurium LT2. Mutat Res 71:25–41

    Google Scholar 

  • Gottesman MM, Gottesman ME, Gottesman S, Gellert M (1974) Characterization of bacteriophage λ reverse as an Escherichia coli phage carrying a unique set of host-derived recombination functions. J Mol Biol 88:471–487

    Google Scholar 

  • Greener A, Hill CW (1980) Identification of a novel genetic element in Escherichia coli K-12. J Bacteriol 144:312–321

    Google Scholar 

  • Hofemeister J, Köhler H, Filippov VD (1979) DNA repair in Proteus mirabilis. VI Plasmid (R-46) mediated recovery and UV-mutagenesis. Mol Gen Genet 176:265–273

    Google Scholar 

  • Kato T (1977) Effects of chloramphenicol and caffeine on postreplication repair in uvrA-umuC and uvrA-recF strain of Escherichia coli K-12. Mol Gen Genet 156:115–120

    Google Scholar 

  • Kato T, Rothman RH, Clark AJ (1977) Analysis of the role of recombination and repair in mutagenesis of Escherichia coli by UV irradiation. Genetics 87:1–18

    Google Scholar 

  • Kato T, Shinoura Y (1977) Isolation and characterization of mutant of Escherichia coli deficient in induction of mutation by ultraviolet light. Mol Gen Genet 156:121–131

    Google Scholar 

  • Kimball RF, Boling ME, Perdue SW (1977) Evidence that UV-inducible error-prone repair is absent in Haemophilus influenzae Rd with a discussion of the relation to error-prone repair of alkylating-agent damage. Mutat Res 44:183–196

    Google Scholar 

  • Lehrbach P, Kung AHC, Lee BTO (1977) Loss of ultraviolet light protection and enhanced ultraviolet ligh — induced mutability in Pseudomonas aeruginosa carrying mutant R-plasmids. J Gen Microbiol 101:135–141

    Google Scholar 

  • Louarn J, Patte J, Jouarn JM (1977) Evidence for a fixed termination site of chromosome replication in Escherichia coli K12. J Mol Biol 115:295–314

    Google Scholar 

  • Louarn J, Patte J, Louarn J (1979) Map position of the replication terminus on the Escherichia coli chromosome. Mol Gen Genet 172:7–11

    Google Scholar 

  • Low B (1973) Restoration by the rac locus of recombinant forming ability in recB- and recC- merozygotes of Escherichia coli K-12. Mol Gen Genet 122:119–130

    Google Scholar 

  • Radman M (1974) Phenomenology of an inducible mutagenic DNA repair pathway in Escherichia coli. In: Molecular and environmental aspects of mutagenesis. Thomas, Springfield Ill, 128–142

    Google Scholar 

  • Radman M (1977) Inducible pathways in deoxyribonucleic acid repair mutagenesis and carcinogenesis. Biochem Soc Trans 5:1194–1196

    Google Scholar 

  • Skavronskaya AG (1978) Influence of plasmids on the reparative activity and UV-induced mutability of bacteria. XIVth International Congress of Genetics. Thesis of reports. Plenary meeting. Symposium, M.35

  • Skavronskaya AG, Andreeva IV, Kondratiev YuS, Aleshkin GI, Abdukhalykova GF, Brukhansky GV, Tiganova IG, Stepanova NF, Demkin VV (1977) Role of plasmids in provision of the capacity of Salmonella to UV-and 4NQO-induced mutability. Dokl Akad Nauk USSR 238:460–463

    Google Scholar 

  • Stepanova NF, Andreeva IV, Stavronskaya AG (1977) Intergeneric mating of Escherichia coli and Salmonella typhimurium. I Construction of Salmonella hybrid with increased recipient ability in crosses with E. coli. Genetica 13:2017–2022

    Google Scholar 

  • Volkert MR, George DL, Witkin EM (1976) Partial suppression of the LexA phenotype by mutations (nmr) which restore ultraviolet resistance but not ultraviolet mutability to Escherichia coli B/r urvA lexA. Mut Res 36:17–28

    Google Scholar 

  • Walker GC (1978) Inducible reactivation and mutagenesis of UV-irradiated bacteriophage P22 in Salmonella typhimurium LT2 containing the plasmid pKM101. J Bacteriol 135:415–421

    Google Scholar 

  • Walsh J, Meynell GG (1967) The effect of various colI factors on the induction of phage P22 by ultraviolet radiation. J Gen Virol 1:587–588

    Google Scholar 

  • Witkin EM (1976) Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli. Bacteriol Rev 40:869–907

    Google Scholar 

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Authors and Affiliations

  1. Gamaleya Institute for Epidemiology and Microbiology, Academy of Medical Sciences, 123098, Moscow, USSR

    A. G. Skavronskaya, N. F. Stepanova & I. V. Andreeva

Authors
  1. A. G. Skavronskaya
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  2. N. F. Stepanova
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  3. I. V. Andreeva
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Communicated by H. Böhme

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Skavronskaya, A.G., Stepanova, N.F. & Andreeva, I.V. UV-mutable hybrids of Salmonella incorporating escherichia coli Region adjacent to Tryptophan operon. Molec. Gen. Genet. 185, 315–318 (1982). https://doi.org/10.1007/BF00330804

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  • DOI: https://doi.org/10.1007/BF00330804

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