Summary
The uptake of L-tyrosine into wild type and antibiotic resistant strains of Schizosaccharomyces pombe requires an energy source, is initially linear with respect to time, is inhibited by 2,4-dinitrophenol and sodium azide and is saturable. However the initial uptake rates and the amount of L-tyrosine accummulated by antibiotic resistant strains are much less than wild type. Comparison of the kinetic constants of uptake shows that mutant strains have a reduced maximum velocity of uptake compared to wild type and a larger Km.
Since the three mutant strains possess a permeability barrier to L-tyrosine as well as being drug resistant this is an indication that antibiotic resistance may be caused by a decrease in plasma membrane permeability.
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References
Anver PR, Griffiths DE (1973) Studies on energy-linked reactions: Resistant mutants of Saccharomyces cerevisiae. Eur J Biochem 32:301–311
Anver PR, Griffiths DE (1973) Studies on energy-linked reactions: Genetic analysis of oligomycin resistant mutants of Saccharomyces cerevisiae. Eur J Biochem 32:312–321
Baranowska H, Polakowska R, Putrament A (1979) Spontaneous and induced non-specific drug resistance in Saccharomyces cerevisiae. Acta Microbiol Ponon 28:181–201
Berry CHJ, Ibrahim MAK, Coddington A (1978) Characterisation of ribosomes from drug resistant strains of Schizosaccharomyces pombe in a Poly U directed cell free protein synthesising system. Mol Gen Genet 167:217–225
Debusk RM, Debusk AG (1980) Physiological and regulatory properties of the general amino acid transport system of Neurospora crassa. J Bacteriol 143:188–197
Foury F, Goffeau A (1975) Stimulation of active uptake of nucleosides and amino acids by cyclic adenosine 3′5′-monophosphate in the yeast Schizosaccharomyces pombe. J Biol Chem 250:2354–2362
Gale EF, Cundliffe E, Reynolds PE, Richmond MH, Waring MJ (1972) The molecular basis of antibiotic action. John Wiley and Sons, London New York Sydney Toronto
Galivan J (1979) Transport and metabolism of methotrexate in normal and resistant cultured rat hepatoma cells. Cancer Res 39:735–743
Gutz H, Heslot H, Leupold U, Loprieno N (1974) In: King RC (ed) Handbook of Genetics, vol 1. Plenum Press, New York and London, pp 395–446
Howell N, Molloy PL, Linnane AW, Lukins HB (1974) Biogenesis of mitochondria 34: The synergistic interaction of nuclear and mitochondrial mutations to produce resistance to high levels of mikamycin in Saccharomyces cerevisiae. Mol Gen Genet 128:43–54
Ibrahim MAK, Coddington A (1976) Genetic studies on cycloheximide resistant strains of Schizosaccharomyces pombe. Heredity 37:179–191
Kotyk A, Rihova L (1972) Transport of α-aminoisobutyric acid in Saccharomyces cerevisiae feedback control. Biochim Biophys Acta 288:380–389
Pestka S (1971) Inhibitors of ribosome functions. Annu Rev Microbiol 25:487–562
Pestka S (1971) Inhibitors of ribosome functions. Annu Rev Biochem 40:697–710
Ramos EH, De Bongioanni LC, Claisse ML, Stoppani AOM (1975) Energy requirements for the uptake of L-leucine by Saccharomyces cerevisiae. Biochim Biophys Acta 394:470–481
Ramos EH, De Bongioanni LC, Cuesta Casado MC, Stoppani AOM (1977) Some properties of L-[14C]leucine transport in Saccharomyces ellipsoideus. Biochim Biophys Acta 467:220–237
Ramos EH, De Bongioanni LC, Stoppani AOM (1980) Kinetics of L-[14C]leucine transport in Saccharomyces cerevisiae. Biochim Biophys Acta 599:214–231
Rank GH, Robertson AJ, Phillips KL (1975) Modification and inheritance of pleiotropic cross resistance and collateral sensitivity in Saccharomyces cerevisiae. Genetics 80:483–493
Rank GH, Bech-Hansen NT (1973) Single nuclear gene inherited cross resistance and collateral sensitivity to 17 inhibitors of mitochondrial function in Saccharomyces cerevisiae. Mol Gen Genet 126:93–102
Rank GH, Robertson A, Phillips K (1975) Reduced plasma membrane permeability in a multiple cross resistant strain of Saccharomyces cerevisiae. J Bacteriol 122:359–366
Rank GH, Gerlach JH, Robertson AJ (1976) Some physiological alterations associated with pleiotropic cross resistance and collateral sensitivity in Saccharomyces cerevisiae. Mol Gen Genet 144:281–288
Rank GH, Robertson AJ, Gerlach JH (1977) Single gene alteration of plasma and mitochondrial membrane function in Saccharomyces cerevisiae. Mol Gen Genet 152:13–18
Subik J, Kovacova V, Takacsova G (1977) Mucidin resistance in yeast: Isolation, characterisation and genetic analysis of nuclear and mitochondrial mucidin resistant mutants of Saccharomyces cerevisiae. Eur J Biochem 73:275–286
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Communicated by F. Kaudewitz
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Johnston, P.A., Coddington, A. Multiple drug resistance in the fission yeast Schizosaccharomyces pombe: Evidence for the existence of pleiotropic mutations affecting energy dependent transport systems. Molec. Gen. Genet. 185, 311–314 (1982). https://doi.org/10.1007/BF00330803
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DOI: https://doi.org/10.1007/BF00330803