Skip to main content
SpringerLink
Log in

Replicon size and mean rate of DNA synthesis in rye (Secale cereale L. cv. Petkus Spring)

  • Published:
Chromosoma Aims and scope Submit manuscript

Abstract

Chromosomal DNA replication was investigated in root meristem cells of Secale cereale L. cv. Petkus Spring using DNA fibre auto-radiography. At 23 ° the mean rate of replication, per single replicon fork, was 12.1 μm/h. Replicon size was between 20–25 μm. These results are compared with corresponding measurements for other angiosperm species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bennett, M.D.: Nuclear DNA content and minimum generation time in herbaceous plants. Proc. roy. Soc. Lond. B 181, 109–135 (1972)

    Google Scholar 

  • Bennett, M.D.: DNA amount, latitude and crop plant distribution. Envir. Exp. Bot. 16, 93–108 (1976)

    Google Scholar 

  • Bennett, M.D.: The time and duration of meiosis. Phil. Trans. Roy. Soc. Lond. B 277, 201–226 (1977)

    Google Scholar 

  • Bennett, M.D., Smith, J.B.: Nuclear DNA amounts in angiosperms. Phil. Trans. roy. Soc. Lond. B 274, 227–274 (1976)

    Google Scholar 

  • Callan, H.G.: Replication of DNA in the chromosomes of eukaryotes. Proc. roy. Soc. Lond. B 181, 19–41 (1972)

    Google Scholar 

  • Edenberg, H.J., Huberman, J.A.: Eukaryotic chromosome replication. Ann. Rev. Genet. 9, 245–284 (1975)

    Google Scholar 

  • Francis, D., Macleod, R.D.: Some cytological changes accompanying the regeneration of meristematic activity at the apex of decapitated roots of Vicia faba L. Ann. Bot. 41, 1149–1162 (1977)

    Google Scholar 

  • Houseman, D., Huberman, J.A.: Changes in the rate of DNA replication fork movement during S phase in mammalian cells. J. Molec. Biol. 94, 173–181 (1975)

    Google Scholar 

  • Huberman, J.A., Riggs, A.O.: On the mechanism of DNA replication in mammalian chromosomes. J. Molec. Biol. 32, 327–341 (1968)

    Google Scholar 

  • Huberman, J.A., Tsai, A.: Direction of DNA replication in mammalian cells. J. Molec. Biol. 75, 5–12 (1973)

    Google Scholar 

  • Lima-de Faria, A.: Differential uptake of tritiated thymidine into hetero- and euchromatin in Melanoplus and Secale. J. Biophys. Biochem. Cytol. 6, 457–466 (1959)

    Google Scholar 

  • McFarlane, P.W., Callan, H.G.: DNA replication in the chromosomes of the chicken, Gallus domesticus. J. Cell Sci. 13, 821–839 (1973)

    Google Scholar 

  • Petes, Th.D., Williamson, D.H.: Fibre autoradiography of replicating yeast DNA. Exp. Cell Res. 95, 103–110 (1975)

    Google Scholar 

  • Taylor, J.H.: DNA synthesis in relation to chromosome reproduction and the reunion of breaks. J. Cell Comp. Physiol. 62, 73–86 (1963)

    Google Scholar 

  • Van't Hof, J.: Relationships between mitotic cycle duration, S period duration and the average rate of DNA synthesis in the root meristem cells of several plants. Exp. Cell Res. 39, 48–58 (1965)

    Google Scholar 

  • Van't Hof, J.: DNA fibre replication in chromosomes of a higher plant (Pisum sativum). Exp. Cell Res. 93, 95–104 (1975)

    Google Scholar 

  • Van't Hof, J.: Replicon size and rate of fork movement in early S of higher plant cells (Pisum sativum). Exp. Cell Res. 103, 395–403 (1976)

    Google Scholar 

  • Van't Hof, J., Bjerknes, C.A.: 18 μm replication units of chromosomal DNA fibers of differentiated cells of pea (Pisum sativum). Chromosoma (Berl.) 64, 287–294 (1977)

    Google Scholar 

  • Van't Hof, J., Bjerknes, C.A.: Similar replicon properties of higher plant cells with different S periods and genome sizes. Exp. Cell Res. 136, 461–465 (1981)

    Google Scholar 

  • Van't Hof, J., Bjerknes, C.A., Clinton, J.H.: Replicon properties of chromosomal DNA fibres and the duration of DNA synthesis of sunflower root-tip meristem cells at different temperatures. Chromosoma (Berl.) 66, 161–171 (1978)

    Google Scholar 

  • Van't Hof, J., Bjerknes, C.A., Clinton, J.H.: Replication of chromosomal DNA fibres of root meristem cells of higher plants. In: Molecular Biology of Plants (H. Smith, ed.), pp. 73–91. New York: Academic Press 1979

    Google Scholar 

  • Van't Hof, J., Kuniyuki, A., Bjerknes, C.A.: The size and number of replicon families of chromosomal DNA of Arabidopsis thaliana. Chromosoma (Berl) 68, 269–285 (1978)

    Google Scholar 

  • Wimber, D.E.: Asynchronous replication of deoxyribonucleic acid in root tip chromosomes of Tradescantia paludosa. Exp. Cell Res. 23, 402–407 (1961)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Plant Science, University College, P.O. Box 78, CF1 1XL, Cardiff, Great Britain

    D. Francis

  2. Plant Breeding Institute, Maris Lane, CB2 2LQ, Trumpington, Cambridge, Great Britain

    M. D. Bennett

Authors
  1. D. Francis
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. D. Bennett
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Francis, D., Bennett, M.D. Replicon size and mean rate of DNA synthesis in rye (Secale cereale L. cv. Petkus Spring). Chromosoma 86, 115–122 (1982). https://doi.org/10.1007/BF00330733

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00330733

Keywords

Search

Navigation