Summary
The ultrastructural localization of peroxidase activity has been studied in the cells of normal human bone marrow using the diaminobenzidine peroxidase technique. Peroxidase activity has been localized within the primary (azurophil) granules of the neutrophilic series as well as in the cytoplasmic granules of eosinophils, basophils and monocytes. Peroxidase activity appears within the cisternal system (nuclear envelope, Golgi complex and rough endoplasmic reticulum) of these cells during the period of peroxidase-containing lysosome production. With the cessation of granulogenesis, peroxidase activity disappears from the cisternal system and does not reappear in subsequent developmental stages. In cells incubated in peroxide-free media, staining of granular components, but not of cisternae, is reduced. The inclusion of catalase in peroxide-free media eliminates all staining. This indicates that an endogenous peroxide is present within the cisternae and granules of these cell types.
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Supported by Grant No. AM-HE-12084-12 from the National Institutes of Health, Bethesda, Maryland.
Appreciation is expressed to Anita Topson and Barbara Jordan for their technical assistance and to Dr. Arthur Sagone who performed the marrow aspirations.
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Ackerman, G.A., Clark, M.A. Ultrastructural localization of peroxidase activity in normal human bone marrow cells. Z. Zellforsch. 117, 463–475 (1971). https://doi.org/10.1007/BF00330708
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DOI: https://doi.org/10.1007/BF00330708