Abstract
Two haemoglobin (Hb) gene clusters cloned from the genomic DNA of Chironomus thummi were localized in the polytene chromosomes of 13 Chironomus species. The haemoglobin gene cluster containing the genes for the monomeric haemoglobin proteins III and IV (λCttG1) hybridized in all species to the end of chromosome arm E. The haemoglobin gene cluster containing the genes for the dimeric HbVIIB proteins (λpiHb1) could be localized to chromosome arm D. The chromosomal position of the haemoglobin genes was always found in morphologically specified groups of bands and is in agreement with cytological data, which have been used to establish the evolutionary relationships between the species of the genus Chironomus.
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Schmidt, E.R., Keyl, H.G. & Hankeln, T. In situ localization of two haemoglobin gene clusters in the chromosomes of 13 species of Chironomus . Chromosoma 96, 353–359 (1988). https://doi.org/10.1007/BF00330701
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DOI: https://doi.org/10.1007/BF00330701