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Genetic mapping of a mutation causing an alteration in Bacillus subtilis ribosomal protein S4

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Summary

A mutation causing an alteration in Bacillus subtilis ribosomal protein S4 was mapped by transformation and PBS-1 transduction to a site between aroG and argA, a region of the B. subtilis chromosome not previously demonstrated to contain ribosomal protein genes. The S4 mutation conferred a spore-plus phenotype in a streptomycinresistant, spore-minus genetic background. The altered protein was detectable by polyacrylamide gel electrophoresis of ribosomal proteins of recombinants scored for the sporeplus phenotype in genetic crosses.

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Authors and Affiliations

  1. Department of Bacteriology and Laboratory of Genetics, University of Wisconsin, 53706, Madison, Wisconsin, USA

    Tina M. Henkin & Glenn H. Chambliss

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  1. Tina M. Henkin
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  2. Glenn H. Chambliss
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Communicated by G.A. O'Donovan

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Henkin, T.M., Chambliss, G.H. Genetic mapping of a mutation causing an alteration in Bacillus subtilis ribosomal protein S4. Molec Gen Genet 193, 364–369 (1984). https://doi.org/10.1007/BF00330694

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  • DOI: https://doi.org/10.1007/BF00330694

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