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Benzyl derivative facilitation of transcription in Escherichia coli at the ara and lac operon promoters: Metabolite gene regulation (MGR)

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Summary

A number of benzyl derivatives have been tested for their ability to induce the expression of the araBAD operon in an Escherichia coli K-12 strain. Those derivatives shown to be stimulatory include: benzoic acid (BA), paramino benzoic acid (PABA), para-hydroxy benzoic acid (PHBA), ortho-amino benzoic acid (OABA), 3-hydroxy-4-methoxy phenylethylamine (MTA), and 4-hydroxy-3-methoxyphenol acetic acid (HVA). The araC gene product was necessary to facilitate the induction. To further characterize if the inductive effect was mediated at the level of transcription, an araBAD-tetracycline resistant (Tcr) operon fusion plasmid (pAP-B) was employed. Benzyl derivatives which induce expression of the araBAD operon in situ also induced a Tcr phenotype with pAP-B. Both indole acetic acid (IAA) and imidazole (IM), which were previously shown to circumvent the necessity for cAMP in the induction of the araBAD operon, also induced a Tcr phenotype with pAP-B. Induction of lac or othe cAMP responding operons with the inducing molecules at the chromosomal level was not detectable when assessed by carbon utilization. However, a lacZYA-Tcr operon fusion plasmid (pLPI) did respond to IAA and several of the inducing benzyl derivatives. Catabolite repression of chromosomal araBAD expression was reversed when the exogenous concentration of OABA was elevated. Similar effects on the Tcr phenotypes conferred by pAP-B and pLP1 were observed when OABA or several other inducing benzyl derivatives were present exogenously.

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Author notes

  1. Robert W. West Jr.

    Present address: Department of Biochemistry and Molecular Biology, 02138, Cambridge, Harvard University, MA, USA

Authors and Affiliations

  1. Department of Microbiology, Clemson University, 29631, Clemson, SC, USA

    Ellis L. Kline, Barbara S. Ink & Priscilla M. Kline

  2. Department of Genetics, University of California, 95616, Davis, CA, USA

    Robert W. West Jr. & Raymond L. Rodriguez

Authors
  1. Ellis L. Kline
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  2. Robert W. West Jr.
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  3. Barbara S. Ink
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  4. Priscilla M. Kline
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  5. Raymond L. Rodriguez
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Communicated by G.A. O'Donovan

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Kline, E.L., West, R.W., Ink, B.S. et al. Benzyl derivative facilitation of transcription in Escherichia coli at the ara and lac operon promoters: Metabolite gene regulation (MGR). Molec Gen Genet 193, 340–348 (1984). https://doi.org/10.1007/BF00330691

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  • DOI: https://doi.org/10.1007/BF00330691

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