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Requirement of protein synthesis for the induction of ribonucleoside diphosphate reductase mRNA in Escherichia coli

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Summary

An RNA-DNA hybridization assay was used to quantitate the ribonucleoside diphosphate reductase mRNA synthesis (nrd mRNA) to show that gene expression was dependent on protein synthesis. The increased nrd mRNA synthesis induced by inhibition of DNA synthesis was eliminated by simultaneous inhibition of protein synthesis. It was further found that protein synthesis is required not only initially but continuously during DNA inhibition for increased expression of nrd mRNA synthesis.

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Authors and Affiliations

  1. Department of Biochemistry, University of Minnesota, College of Biological Sciences, 55108, St. Paul, MN, USA

    Paul D. Hanke & James A. Fuchs

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  1. Paul D. Hanke
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  2. James A. Fuchs
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Communicated by G.A. O'Donovan

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Hanke, P.D., Fuchs, J.A. Requirement of protein synthesis for the induction of ribonucleoside diphosphate reductase mRNA in Escherichia coli . Molec Gen Genet 193, 327–331 (1984). https://doi.org/10.1007/BF00330689

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  • DOI: https://doi.org/10.1007/BF00330689

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