Summary
The inheritance of the high molecular weight (HMW) glutenins and of several gliadins controlled, respectively, by the long and short arms of chromosome 1B of common wheat was studied. Analysis was carried out on the progeny of two inter-varietal crosses in which the parental lines possessed differentially migrating subunits as revealed by sodium dodecyl sulphate polyacrylamide gel electrophoresis. No recombination event was detected either within the fraction of the HMW glutenins or among most of the gliadin subunits studied indicating that they are controlled by tightly linked gene clusters. One gliadin subunit (B30) showed 25.5% recombination frequency with the rest of the gliadin subunits and 23.5% recombination frequency with the fraction of the HMW glutenin subunits. It has been concluded that this subunit is controlled by a separate locus (Gld-B6), proximal to the major gliadin gene cluster on the short arm of chromosome 1B. Consequently, the recombination percentage between the glutenin loci and most of the gliadin loci was calculated as 49.0 and the distance in centi-Morgans (cM) as 53.6. The estimated distance in cM is very close to the observed recombination percentage. A genetic map of these storage protein genes is presented.
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References
Bietz JA, Shepherd KW, Wall JS (1975) Single kernel analysis of glutenin: use in wheat genetics and breeding. Cereal Chem 52:513–532
Blake TK, Ullrich SE, Nilan RA (1982) Mapping of the Hor-3 locus encoding D hordeins in barley. Theor Appl Genet 63:367–371
Brown JWS, Kemble RJ, Law CN, Flavell RB (1979) Control of endosperm protein in Triticum aestivum (var. Chinese Spring) and Aegilops umbellulata by homoeologous group 1 chromosomes. Genetics 93:189–200
Brown JWS, Law CN, Worland AJ, Flavell RB (1981) Genetic variation in wheat endosperm proteins: an analysis by twodimensional electrophoresis using intervarietal chromosomal substitution lines. Theor Appl Genet 59:361–371
Damidaux R, Autran J-C, Grignac P, Feillet P (1980) Déterminisme génétique des constituants gliadines de Triticum durum Desf associés à la qualité culinaire intrinsèque des varieties. CR Acad Sci (Paris) 291: Sèrie D: 585–588
Doll H, Brown ADH (1979) Hordein variation in wild Hordeum spontaneum and cultivated (H. vulgare) barley. Can J Genet Cytol 21:391–404
Field JM, Shewry PR, March JF, Miflin BJ (1982) The purification and characterization of homologous high molecular weight storage proteins from grain of wheat, rye and barley. Theor Appl Genet 62:329–336
Galili G, Feldman M (1983a) Genetic control of endosperm proteins in wheat. I. The use of high resolution one-dimensional gel electrophoresis for the allocation of genes coding for endosperm protein subunits in the common wheat cultivar Chinese Spring. Theor Appl Genet 64:97–101
Galili G, Feldman M (1983b) Genetic control of endosperm proteins in wheat. II. Variation of high molecular weight glutenin and gliadin subunits of Triticum aestivum. Theor Appl Genet 66:77–86
Islam AKMR, Shepherd KW (1981) Wheat-barley addition lines: their use in genetic and evolutionary studies of barley. Proc 4th Int Barley Genet Symp, Edinburgh, pp 729–739. University Press, Edinburgh
Jensen J, Jorgensen JH, Jensen HP, Giese H, Doll H (1980) Linkage of the hordein loci, Hor 1 and Hor 2 with the powdery mildew resistance loci Ml-k and Ml-a on barley chromosome 5. Theor Appl Genet 58:27–31
Kasarda DD, Bernardin JE, Nimmo CC (1976a) Wheat proteins. In: Pomeranz Y (ed) Advances in cereal science and technology. Am Assoc, Cereal Chem, St. Paul, Minnesota, pp 158–236
Kasarda DD, Bernardin JE, Qualset CO (1976b) Relationship of gliadin protein components to chromosomes in hexaploid wheats (Triticum aestivum I.). Proc Natl Acad Sci USA 73:3646–3650
Khan K, Bushuk W (1977) Studies of glutenin. IX. Subunit composition by sodium dodecyl sulfate-polyacrylamide gel electrophoresis at pH 7.3 and 8.9. Cereal Chem 54:588–596
Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175
Lawrence GJ, Shepherd KW (1980) Variation in glutenin protein subunits of wheat. Aust J Biol Sci 33:221–233
Lawrence GJ, Shepherd KW (1981) Inheritance of glutenin protein subunits in wheat. Theor Appl Genet 60:333–337
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680–685
Mecham DK, Kasarda DD, Qualset CO (1978) Genetic aspects of wheat gliadin proteins. Biochem Genet 16:831–853
Miflin BJ, Shewry PR (1979) The biology and biochemistry of cereal seed prolamins. In: Seed protein improvement in cereals and grain legumes, vol 1. International Atomic Energy Agency, Vienna, p 137
Payne PI, Law CN, Mudd EE (1980) Control by homoeologous group 1 chromosomes of the high-molecular-weight subunits of glutenin, a major protein of wheat endosperm. Theor Appl Genet 58:113–120
Payne Pl, Corfield KG, Holt LM, Blackman JA (1981a) Correlations between the inheritance of certain high-molecular-weight subunits of glutenin and bread-making quality in progenies of six crosses of bread wheat. J Sci Food Agric 32:51–60
Payne PI, Holt LM, Law CN (1981b) Structural and genetical studies on the high-molecular-weight subunits of wheat glutenin. Part 1: Allelic variation in subunits amongst varieties of wheat (Triticum aestivum). Theor Appl Genet 60:229–236
Payne PI, Holt LM, Worland AJ, Law CN (1982) Structural and genetical studies on the high-molecular-weight subunits of wheat glutenin. Part 3. Telocentric mapping of the subunit genes on the long arms of the homoeologous group 1 chromosomes. Theor Appl Genet 63:129–138
Powling A, Islam AKMR, Shepherd KW (1981) Isozymes in wheat barley hybrid derivative lines. Biochem Genet 19:237–254
Rybalka AI, Sozinov AA (1979) Mapping the locus of Gld 1B, which controls the biosynthesis of reserve proteins in soft wheat. Tsitologiyai Genetika 13:276–282
Shewry PR, Pratt HM, Finch RA, Miflin BJ (1978) Genetic analysis of hordein polypeptides from single seeds of barley. heredity 40:463–466
Shewry PR, Autran JC, Nimmo CC, Lew EJ-L, Kasarda DD (1980a) N-terminal amino acid sequence of storage protein components from barley and diploid wheat. Nature 286:520–522
Shewry PR, Faulks AJ, Pickering RA, Jones IT, Finch RA, Miflin BJ (1980b) The genetic analysis of barley storage proteins. Heredity 44:383–389
Sozinov AA, Poperelya FA (1980) Genetic classification of prolamines and its use for plant breeding. Ann Technol Agric 29:229–245
Wall JS (1979) The role of wheat proteins in determning baking quality. In: Laidman DL, Wyn Jones RG (eds) Recent advances in the biochemistry of cereals. Academic Press, New York, pp 275–311
Wrigley CW, Shepherd KW (1973) Electrofocusing of grain proteins from wheat genotypes. Ann NY Acad Sci 209:154–162
Wrigley CW, Lawrence GJ, Shepherd KW (1982) Association of glutenin subunits with gliadin composition and grain quality in wheat. Aust J Plant Physiol 9:15–30
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Communicated by G. A. O'Donovan
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Galili, G., Feldman, M. Mapping of glutenin and gliadin genes located on chromosome 1B of common wheat. Molec Gen Genet 193, 293–298 (1984). https://doi.org/10.1007/BF00330683
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DOI: https://doi.org/10.1007/BF00330683