Summary
F42lac recombination with λplac is normally 20-fold to 50-fold higher than recombination between λplac and a chromosomal lac gene. Transductional crosses with λplac and a recombinant plasmid containing the lac operon and F replication functions show only two to four-fold higher recombination than similar crosses with a chromosomal lac gene. Insertion of a BamHI fragment containing the entire tra regulon of F into the mini-F-lac plasmid restores the high level of recombination seen with F42lac.
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Communicated by G.A. O'Donovan
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Seifert, H.S., Porter, R.D. Enhanced recombination between λplac5 and mini-F-lac: The tra regulon is required for recombination enhancement. Molec Gen Genet 193, 269–274 (1984). https://doi.org/10.1007/BF00330679
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DOI: https://doi.org/10.1007/BF00330679