Summary
To get an idea about the response of a living system, exposed to gradually increasing doses of a mutagen for several generations, a population of V-79 Chinese hamster cells was exposed repeatedly to gradually increasing doses of UV radiation. Each dose was followed by a variable period of growth for at least ten generations. After treatment the cells were not mutable by UV radiation, though MNNG was capable of producing mutations with the same efficiency as in the untreated cells. In terms of viability, the treated cells behaved exactly as the untreated ones for both UV and MNNG. The observed behaviour of the treated cells was found to be stable for during the 50 passages studied.
Similar content being viewed by others
Abbreviations
- DMSO:
-
dimethylsulphoxide
- Aza:
-
8-Azaguanine
- MEM:
-
minimal essential medium
- PBS:
-
phosphate buffered saline
- MNNG:
-
N-methyl-N′-nitro-N-nitrosoguanidine
References
Bhattacharjee SB, Pal B (1982) Tetracycline induced mutations in Chinese hamster cells. Mutat Res 101:329–338
Bhattacharjee SB, Chatterjee S, Pal B (1982) Survival and mutation of Chinese hamster cells after ultraviolet irradiation and caffeine treatment. Mutat Res 106:137–146
Dasgupta UB, Summers WC (1978) Ultraviolet reactivation of herpes simplex virus is mutagenic and inducible in mammalian cells. Proc Natl Acad Sci USA 75:2378–2381
Eagle H (1959) Amino acid metabolism in mammalian cell cultures. Science 130:432–437
Fox M, McMillan S (1978) Evidence for the involvement of different repair mechanisms in mutagenesis and cell killing in V-79 cells. In: Hanawalt PC, Friedberg EC, Fox CF (eds) DNA repair mechanisms. Academic Press, New York, p 723
Hatchard CG, Parker CA (1956) A new sensitive chemical actinometer, II. Potasssium ferrioxalate as a standard chemical actinometer. Proc Roy Soc (London) A 235:518–536
Jeggo P, Defais M, Samson L, Schendel P (1977) An adaptive response of E. coli to low levels of alkylating agent: Comparison with previously characterized DNA repair pathways. Mol Gen Genet 157:1–9
Jeggo P, Defais M, Samson L, Schendel P (1978) The adaptive response of E. coli to low levels of alkylating agent: The role of pol A in killing adaptation. Mol Gen Genet 162:299–305
Kaina B (1982) Enhanced survival and reduced mutation and aberration frequencies induced in V-79 Chinese hamster cells preexposed to low levels of methylating agents. Mutat Res 93:195–211
Radman M (1974) Phenomenology of an inducible mutagenic DNA repair pathway in Escherichia coli: SOS repair hypothesis. In: Prakash L, Sherman F, Miller M, Lawrence C, Tabor HW (eds) Molecular and Environmental Aspects of Mutagenesis. Thomas, Springfield, IL, p 128
Radman M (1980) Is there SOS induction in mammalian cells? Photochem Photobiol 32:823–830
Samson L, Cairns J (1977) A new pathway for DNA repair in Escherichia coli. Nature 267:281–283
Samson L, Schwartz JL (1980) Evidence for an adaptive DNA repair pathway in CHO and human skin fibroblast cell lines. Nature 287:861–863
Witkin EM (1976) Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli. Bacteriol Rev 40:869–907
Author information
Authors and Affiliations
Additional information
Communicated by G.A. O'Donovan
Rights and permissions
About this article
Cite this article
Chatterjee, S., Bhattacharjee, S.B., Bhattacharyya, N. et al. UV non-mutable mutant from V-79 Chinese hamster cells. Molec Gen Genet 193, 251–254 (1984). https://doi.org/10.1007/BF00330676
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00330676