Summary
We have previously shown that DNA gyrase of Escherichia coli can promote recombination between heterologous DNAs in a cell-free system (Ikeda et al. 1982). In the present paper, we have studied the nucleotide sequences of several recombination junctions of λ-pBR322 recombinants and found that there were not more than three-basepair homologies between the parental DNAs in six combinations of the λ and pBR322 recombination sites. Based on this and previous results, we concluded that homology was not required for the DNA gyrase-mediated recombination. Furthermore, the structures of the recombinant DNAs we have analyzed suggest the occurrence of multiple crossovers in our in vitro system.
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Naito, A., Naito, S. & Ikeda, H. Homology is not required for recombination mediated by DNA gyrase of Escherichia coli . Molec Gen Genet 193, 238–243 (1984). https://doi.org/10.1007/BF00330674
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DOI: https://doi.org/10.1007/BF00330674