Summary
Synthesis of both chromosomal and plasmid (pBR322) DNA was measured in E. coli strains differing in their relA allele (relA +: CP78; relA: CP79). It was found that upon limitation of a required amino acid or after valine treatment to trigger a stringent response synthesis of pBR322 DNA was stimulated only in the relaxed strain and was inhibited in its stringent counterpart. The results suggest that replication of plasmid DNA is negatively controlled by the relA + allele.
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References
Cashel M, Gallant J (1969) Two compounds implicated in the function of the RC gene of Escherichia coli. Nature 221:838–841
Cashel M, Lazzarini RA, Kalbacher R (1969) An improved method for thin-layer chromatography of nucleotide mixtures containing 32P-labeled orthophosphate. J Chromatogr 40:103–109
Clewell DB (1972) Nature of ColE1 plasmid replication in Escherichia coli in the presence of chloramphenicol. J Bacteriol 110:667–676
Clewell DB, Helinski DR (1972) Effect of growth conditions on the formation of the relaxation complex of supercoiled ColE1 deoxyribonucleic acid and protein in Escherichia coli. J Bacteriol 110:1135–1146
Gallant JA (1979) Stringent control in E. coli. Annu Rev Genet 13:393–415
Hecker M, Prösch S, Mach H, Mach F (1981) Synthese Poly(A)-haltiger RNA in auswachsenden Sporen von Bacillus subtilis. Z Allg Mikrobiol 21:35–40
Hecker M, Wiehle W, Schroeter A, Mach F (1983) Expression des Plasmids pBR322 während diskontinuierlicher Kultivierung eines stringent und relaxed kontrollierten Stammes von Escherichia coli. Z Allg Mikrobiol 23:Heft 4
Hochmannova J, Nesvera J (1982) A rapid and economic method for estimation of the number of plasmid copies in Escherichia coli cells. Folia Microbiol 27:138–141
Itoh T, Tomizawa J (1980) Formation of an RNA primer for initiation of replication of ColE1 DNA by ribonuclease H. Proc Natl Acad Sci USA 77:2450–2454
Lark KG (1969) Initiation and control of DNA synthesis. Annu Rev Biochem 38:569–604
Marsh RC, Hepburn ML (1980) Initiation and termination of chromosome replication in Escherichia coli subjected to amino acid starvation. J Bacteriol 142:236–242
Mitchell JJ, Lucas-Lenard JM (1980) The effect of alcohol on guanosine-5′-diphosphate-3′-diphosphate metabolism in stringent and relaxed Escherichia coli. J Biol Chem 255:6307–6313
Travers A, Kari C, Mace H (1981) Transcriptional regulation by bacterial RNA polymerase. In: Genetics as a tool in microbiology. Cambridge University Press, Cambridge, England, pp 113–130
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Communicated by H. Böhme
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Hecker, M., Schroeter, A. & Mach, F. Replication of pBR322 DNA in stringent and relaxed strains of Escherichia coli . Mol Gen Genet 190, 355–357 (1983). https://doi.org/10.1007/BF00330665
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DOI: https://doi.org/10.1007/BF00330665