Summary
When E. coli cells carrying the plasmid pLG13 (coding for the newly discovered type II restriction endonuclease EcoRV) are infected with phage T3 or T7, only T7 is able to replicate normally. T3 wild-type as well as its ocr − mutants are subject to DNA restriction in vivo and in vitro. The EcoRV enzyme cuts T3 DNA at 5 sites. T7 and its ocr − mutants have no EcoRV sites in their DNA. In contrast to the anti-restriction activity of the T3 and T7 ocr + gene function against type I and III restriction enzymes, the ocr + protein is unable to inactivate the type II restriction endonuclease EcoRV.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- EcoB:
-
EcoK, EcoP1, EcoRV, HaeIII, HincII, HpaI, HpaII, restriction endonucleases coded by Escherichia coli strains B or K, E. coli plasmids P1 or pLG13, Haemophilus aegypticus, H. influenzae serotype c, and H. parainfluenzae, resp
- e.o.p.:
-
efficiency of plating
- gp:
-
gene product (in the sense of protein)
- kb:
-
kilobases, base pairs x 103
- ocr + :
-
gene function which overcomes classical restriction
- SAM:
-
S-adenosylmethionine
- sam + :
-
gene function with S-adenosylmethionine hydrolase (SAMase) activity
References
Bailey JN, Dembinski DR, McAllister WT (1980) Derivation of a restriction map of bacteriophage T3 DNA and its comparison with the map of bacteriophage T7 DNA. J Virol 35:176–183
Bickle TA (1982) The ATP-dependent restriction endonucleases. In: Linn SM and Roberts RJ (eds) The Nucleases. Cold Spring Harbor Press, New York, p 85–108
Duckworth DH, Glenn J, McCorquodale DJ (1981) Inhibition of bacteriophage replication by extrachromosomal genetic elements. Microbiol Rev 45:52–71
Dunn JJ, Studier FW (1981) Nucleotide sequence from the genetic left end of bacteriophage T7 DNA to the beginning of gene 4. J Mol Biol 148:303–330
Glatman LI, Moroz AF, Yablokova MB, Rebentish BA, Kholmina GV (1980) A novel plasmid-mediated DNA restrictionmodification system in E. coli. Plasmid 4:350–351
Kholmina GV, Rebentish BA, Skoblov YS, Mironov AA, Yankovski NK, Kozlov YI, Glatman LI, Moroz AF, Debabov VG (1980) Isolation and characterization of a new site-specific endonuclease EcoRV (in Russian). Dokl Akad Nauk SSSR 253:495–497
Krüger DH, Schroeder C (1981) Bacteriophage T3 and bacteriophage T7 virus-host cell interactions. Microbiol Rev 45:9–51
Krüger DH, Schroeder C, Hansen S, Rosenthal HA (1977) Active protection by bacteriophages T3 and T7 against E. coli B- and K-specific restriction of their DNA. Mol Gen Genet 153:99–106
Krüger DH, Gola G, Weisshuhn I, Hansen S (1978) The ocr gene function of bacterial viruses T3 and T7 prevents host-controlled modification. J Gen Virol 41:189–192
Krüger DH, Hansen S, Schroeder C (1980) Host-dependent modification of bacterial virus T3 affecting its adsorption ability. Virology 102:444–446
Krüger DH, Reuter M, Hansen S, Schroeder C (1982) Influence of phage T3 and T7 gene functions on a type III (EcoP1) DNA restriction-modification system in vivo. Mol Gen Genet 185:457–461
Mark KK, Studier FW (1981) Purification of the gene 0.3 protein of bacteriophage T7, an inhibitor of the DNA restriction system of E. coli. J Biol Chem 256:2573–2578
McAllister WT, Carter AD (1980) Regulation of promoter selection by the bacteriophage T7 RNA polymerase in vitro. Nucl Acids Res 8:4821–4837
Modrich P (1982) Studies on sequence recognition by type II restriction and modification enzymes. Crit Rev Biochem 13:287–323
Rosenberg AH, Simon MN, Studier FW, Roberts RJ (1979) Survey and mapping of restriction endonuclease cleavage sites in bacteriophage T7 DNA. J Mol Biol 135:907–915
Stahl SJ, Zinn K (1981) Nucleotide sequence of the cloned gene for bacteriophage T7 RNA polymerase. J Mol Biol 148:481–485
Studier FW (1979) Relationships among different strains of T7 and among T7-related bacteriophages. Virology 95:70–84
Author information
Authors and Affiliations
Additional information
Communicated by W. Arber
Rights and permissions
About this article
Cite this article
Krüger, D.H., Reuter, M., Schroeder, C. et al. Restriction of bacteriophage T3 and T7 ocr + strains by the type II restriction endonuclease EcoRV. Mol Gen Genet 190, 349–351 (1983). https://doi.org/10.1007/BF00330663
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00330663