Summary
Five recombinant plasmids, pBK2646, pBK611, pRC3, pRC4 and pRC5, carrying rpoB rifampicin-resistant RNA-polymerase genes were obtained.
The sequence analysis of these plasmids revealed certain structural changes in the rpoB gene which specify corresponding alterations in the β-subunit of RNA polymerase. Some functional properties of the corresponding mutant strains and their RNA polymerases have been investigated.
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Communicated by D. Goldfarb
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Ovchinnikov, Y.A., Monastyrskaya, G.S., Guriev, S.O. et al. RNA polymerase rifampicin resistance mutations in Escherichia coli: Sequence changes and dominance. Mol Gen Genet 190, 344–348 (1983). https://doi.org/10.1007/BF00330662
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DOI: https://doi.org/10.1007/BF00330662