Skip to main content
SpringerLink
Log in

Induction in Saccharomyces cerevisiae of mitotic recombination by mono and bifunctional agents: Comparison of the pso 2-1 and rad52 repair deficient mutants to the wild-type

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

The induction in Saccharomyces cerevisiae of mitotic gene conversion and crossing-over by photoaddition of mono and bifunctional psoralen derivatives as well as by mono (HN1) and bifunctional (HN2) nitrogen mustards or 254 nm ultraviolet (UV) radiation was compared in wild type and in a mutant, pso2-1, initially selected for sensitivity to DNA cross-linking agents. The induction of the same recombinational events by photoaddition of one mono and one bifunctional derivative of psoralen was also examined in the rad52-1 diploid strain.

The non-reciprocal (gene conversion) and the reciprocal (crossing-over) events follow the same patterns for each agent tested regarding the relative sensitivities of the wild type and repair-deficient strains.

The pso2-1 strain retains the same or even a greater ability to perform recombination than the wild type following exposure to monofunctional agents, as well as to HN2 and UV. In contrast, this mutant is not able to perform mitotic recombination as efficiently as the wild type specifically after photoaddition of bifunctional furocoumarins. Such a difference is not simply due to the relative toxicity of the inducing agents.

The rad52-1 mutant is blocked in induced recombination by the photoaddition of both the mono and bifunctional furocoumarins tested and the same was already established for UV and γ-rays.

The rad52-1 and pso2-1 mutant strains have in common a high sensitivity in the G2 phase of the cell cycle and a loss of the ability to restitute high molecular weight DNA after the appearance of DNA double strand breaks related to the incision of inter-strand cross-links. This leads to a generalised block in recombination ability in rad52 whereas in the pso2-1 mutant the nature of the deficiency in recombination is much more specific.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Abel G, Schimmer O (1981) Mutagenicity and toxicity of furocoumarins: comparative investigations in two test systems. Mutat Res 90:451–461

    Google Scholar 

  • Averbeck D, Moustacchi E (1975) 8-methoxypsoralen plus 365 nm light effects and repair in yeast. Biochim Biophys Acta 395:393–404

    Google Scholar 

  • Averbeck D, Moustacchi E (1979) Genetic effect of 3-carbethoxypsoralen, angelicin, psoralen and 8-methoxypsoralen + 365 nm irradiation in Saccharomyces cerevisiae. Induction of reversions, mitotic crossing-over, gene conversion and cytoplasmic ‘petite’ mutations. Mutat Res 68:133–148

    Google Scholar 

  • Averbeck D, Moustacchi E (1980) Decreased photo-induced mutagenicity of monofunctional as opposed to bi-functional furocoumarins in yeast. Photochem Photobiol 31:475–478

    Google Scholar 

  • Averbeck D, Moustacchi E, Bisagni E (1978) Biological effects of damages photoinduced by a derivative of psoralen substituted at the 3,4 reaction site. Photoreactivity of this compound and lethal effect in yeast. Biochim Biophys Acta 518:464–481

    Google Scholar 

  • Brendel M, Haynes RH (1973) Interaction among genes controlling sensitivity to radiation and alkylation in yeast. Mol Gen Genet 125:197–216

    Google Scholar 

  • Bridges BA, Mottershead RP, Knowles A (1979) Mutation induction and killing of Escherichia coli by DNA adducts and crosslinks: a photobiological study with 8-methoxypsoralen. Chem Biol Interactions 27:221–233

    Google Scholar 

  • Burger PM, Simons JWIM (1979) Mutagenicity of 8-methoxypsoralen and long-ware ultraviolet irradiation in V-79 chinese hamster cells. A first approach to a risk estimate in photochemotherapy. Mutat Res 60:381–389

    Google Scholar 

  • Cassier C, Chanet R, Henriques JAP, Moustacchi E (1980) The effects of three pso genes on induced mutagenesis: A novel class of mutationally defective yeast. Genetics 96:841–857

    Google Scholar 

  • Cassier C, Moustacchi E (1981) Mutagenesis induced by mono-and bi-functional alkylating agents in yeast mutants sensitive to photo-addition of furocoumarins (pso). Mutat Res 84:37–47

    Google Scholar 

  • Cassier C (1982) Contrôle génétique de la mutabilité induite et de la conversion induite chez la levure; influence des gènes pso qui gouvernent la sensibilité à la photoaddition des furocoumarines. Thèse de 3ème cycle, Université Paris-Sud, Orsay

  • Cassuto E, Gros N, Bardwell E, Howard-Flanders P (1977) Genetic effects of photoadducts and photocross-links in the DNA of phage exposed to 360 nm light and trimethyl psoralen or khellin. Biochem Biophys Acta 475:589–600

    Google Scholar 

  • Chlebowicz E, Jachymczyk W (1979) Repair of MMS-induced DNA double-strand breaks in haploid cells of Saccharomyces cerevisiae, which requires the presence of a duplicate genome. Mol Gen Genet 167:279–280

    Google Scholar 

  • Eckardt F, Haynes R (1980) Quantitative measures of mutagenicity and mutability based on mutant yield data. Mutat Res 74:439–458

    Google Scholar 

  • Fox M, Scott D (1980) The genetic toxicology of nitrogen and sulphur mustard. Mutat Res 75:131–168

    Google Scholar 

  • Fukuhara H (1967) Protein synthesis in non-growing yeast. The respiratory adaptation system. Biochim Biophys Acta 134:143–164

    Google Scholar 

  • Game JC, Zamb TJ, Braun RJ, Resnick MA, Roth RM (1980) The role of radiation (rad) genes in meiotic recombination in yeast. Genetics 94:51–68

    Google Scholar 

  • Grant EL, von Borstel RC, Ashwood-Smith MJ (1979) Mutagenicity of cross-links and monoadducts of furocoumarins (psoralen and angelicin) induced by 360 nm radiation in excision-repair defective and radiation-insensitive strains of Saccharomyces cerevisiae. Environmental Mutagenesis 1:55–63

    Google Scholar 

  • Hama-Inaba H, Hieda-Shiomi N, Shiomi T, Sato K (1983) Isolation and characterization of mitomycin-C-sensitive mouse lymphoma mutants. Mutat Res 108:405–416

    Google Scholar 

  • Haynes RH, Kunz BA (1981) DNA repair and mutagenesis in yeast. In: Strathen JN, Jones EW, Broach JR (eds) The molecular biology of the yeast. Life cycle and inheritance. Cold Spring Harbor Publication, pp 371–414

  • Henriques JAP, Moustacchi E (1980a) Sensitivity to photoaddition of mono- and bifunctional furocoumarins of X-ray sensitive mutants of Saccharomyces cerevisiae. Photochem Photobiol 31:557–563

    Google Scholar 

  • Henriques JAP, Moustacchi E (1980b) Isolation and characterization of pso mutants sensitive to photoaddition of psoralen derivatives in Saccharomyces cerevisiae. Genetics 95:273–288

    Google Scholar 

  • Henriques JAP, Moustacchi E (1981) Interactions between mutations for sensitivity to psoralen photoaddition (pso) and to radiation (rad) in Saccharomyces cerevisiae. J Bacteriol 148:248–256

    Google Scholar 

  • Isaacs ST, Shen CKJ, Hearst JE, Rapoport H (1977) Synthesis and characterization of new psoralen derivatives with superior photoreactivity with DNA and RNA. Biochemistry 16:1058–1064

    Google Scholar 

  • Jachymczyk W, von Borstel RC, Mowat MRA, Hastings PJ (1981) Repair of interstrand cross-links in DNA of Saccharomyces cerevisiae requires two systems for DNA repair: the RAD3 system and the RAD51 system. Mol Gen Genet 182:196–205

    Google Scholar 

  • Kato T, Shinoura Y (1977) Isolation and characterization of mutants of E. coli deficient in induction of mutations by ultraviolet light. Mol Gen Genet 156:121–131

    Google Scholar 

  • Kunz BA, Haynes RH (1981) Phenomenology and genetic control of mitotic recombination in yeast. Annu Rev Genet 15:57–89

    Google Scholar 

  • Lawrence CW (1981) Mutagenesis in Saccharomyces cerevisiae. Adv Genet 21: In press

  • Lawrence CW, Christensen R (1976) UV mutagenesis in radiationsensitive strains of yeast. Genetics 82:207–232

    Google Scholar 

  • Lemontt JF (1971) Mutants of yeast defective in mutation induced by ultraviolet light. Genetics 68:21–33

    Google Scholar 

  • Lemontt JF (1980) Genetic and physiological factors affecting repair and mutagenesis in yeast. In: Genoroso WM, Shelby MD, de Serres FJ (eds) DNA repair and mutagenesis in eukaryotes. Plenum Press, New York, pp 85–179

    Google Scholar 

  • Machida I, Nakai S (1980) Induction of spontaneous and UV-induced mutations during commitment to meiosis in Saccharomyces cerevisiae. Mutat Res 73:59–68

    Google Scholar 

  • Machida I, Saeki T, Nakai S (1977) Genetic pathway of radiation-induced mitotic recombination in yeast. Jpn J Genet 52:455

    Google Scholar 

  • Magaña-Schwencke N, Averbeck D, Henriques JAP, Moustacchi E (1980) Absence de pontages inter-chaines dans l'ADN traité par le 3-carbéthoxypsoralène et une irradiation à 365 nm. CR Acad Sci Paris 291:207–210

    Google Scholar 

  • Magaña-Schwencke N, Henriques JAP, Chanet R, Moustacchi E (1982) The fate of 8-methoxypsoralen photoinduced cross-links in nuclear and mitochondrial yeast DNA: Comparison of wildtype and repair-deficient strains. Proc Natl Acad Sci USA 79:1722–1726

    Google Scholar 

  • Malone RF, Easton-Esposito R (1980) The RAD 52 gene is required for homothallic interconversion of mating types and spontaneous mitotic recombination in yeast. Proc Natl Acad Sci USA 77:503–507

    Google Scholar 

  • Mc Kee R, Lawrence CW (1979) Genetic analysis of γ-ray mutagenesis in yeast. I. Reversion in radiation-sensitive strains. Genetics 93:361–373

    Google Scholar 

  • Musajo L, Rodighiero GR, Caporale G, Dall'acqua F, Marciani S, Bordin F, Baccichetti F, Bevilacqua R (1974) Photoreactions between skin-photosensitizing furocoumarins and nucleic acids. In: Fitzpatrick TB, Pathak MA, Harber LC, Seiji M, Kukita A (eds) Sunlight and man. University of Tokyo Press, Tokyo, pp 369–387

    Google Scholar 

  • Moustacchi E (1969) Cytoplasmic and nuclear genetic events induced by UV light in strains of Saccharomyces cerevisiae with different UV sensitivities. Mutat Res 4:129–136

    Google Scholar 

  • Parsons BJ (1980) Psoralen photochemistry. Photochem Photobiol 32:813–821

    Google Scholar 

  • Prakash L, Prakash S (1977) Isolation and characterization of MMS sensitive mutants of Saccharomyces cerevisiae. Genetics 86:33–55

    Google Scholar 

  • Prakash S, Prakash L, Burke W, Montelone BA (1980) Effects of the RAD 52 gene on recombination in Saccharomyces cerevisiae. Genetics 94:31–50

    Google Scholar 

  • Prakash L, Taillon-Miller P (1981) Effects of the rad52 gene on sister chromatid recombination in Saccharomyces cerevisiae. Curr Genet 3:247–250

    Google Scholar 

  • Resnick MA (1975) The repair of double-strand breaks in chromosomal DNA of yeast. In: Hanawalt PC, Setlow RB (eds) Molecular mechanisms for repair of DNA, part B. Plenum Press, New York, pp 549–556

    Google Scholar 

  • Resnick MA, Martin P (1976) The repair of double-strand breaks in nuclear DNA of Saccharomyces cervisiae and its genetic control. Mol Gen Genet 143:119–129

    Google Scholar 

  • Ruhland A, Brendel M (1979) Mutagenesis by cytostatic alkylating agents in yeast strains of differing repair capacities. Genetics 92:83–97

    Google Scholar 

  • Ruhland A, Haase E, Siede W, Brendel M (1981) Isolation of yeast mutants sensitive to the bifunctional alkylating agent nitrogen mustard. Mol Gen Genet 181:346–351

    Google Scholar 

  • Saeki T, Machida I, Nakai S (1980) Genetic control of diploid recovery after γ-irradiation in the yeast Saccharomyces cerevisiae. Mutat Res 73:251–265

    Google Scholar 

  • Sato K, Hieda N (1979a) Isolation of a mammalian cell mutant sensitive to 4-nitroquinoleine-1-oxide. Int J Radiat Biol 35:83–87

    Google Scholar 

  • Sato K, Hieda N (1979b) Isolation and characterization of a mutant mouse lymphoma cell sensitive to methyl methanesulfonate and X-rays. Radiat Res 78:167–171

    Google Scholar 

  • Sato K, Hieda N (1980) Mutation induction in a mouse lymphoma cell mutant sensitive to 4-nitroquinleine-1-oxide and ultraviolet radiation. Mutat Res 71:233–241

    Google Scholar 

  • Schimmer O, Beck R, Dietz U (1980) Phototoxizität und Photomutagenität von Furocumarinen bei Chlamydomonas reinhardii Vergleichende Untersuchungen zur biologischen Aktivität als Grundlage für eine Riskoabschätzung. Planta Med 40:68–76

    Google Scholar 

  • Shiomi T Sato K (1979) Isolation of UV-sensitive variants of human FL cells by a viral suicide method. Somatic Cell Genet 5:193–201

    Google Scholar 

  • Shiomi T, Hieda-Shiomi N, Sato K, Tsuji H, Takahashi E, Tobari I (1981) A mouse-cell mutant sensitive to ionizing radiation is hypermutable by low doses of γ-irradiation. Mutat Res 83:107–116

    Google Scholar 

  • Scott BR, Pathak MA, Mohn GR (1976) Molecular and genetic basis of furocoumarin reactions. Mutat Res 39:29–74

    Google Scholar 

  • Siede W, Brendel M (1982) Interaction among genes controlling sensitivity to radiation (RAD) and to alkylation by nitrogen mustard (SNM) in yeast. Curr Genet 5:33–38

    Google Scholar 

  • Song PS, Tapley KJ (1979) Photochemistry and photobiology of psoralens. Photochem Photobiol 29:1177–1197

    Google Scholar 

  • Strike P, Wilbraham HO, Seeberg E (1981) Repair of psoralen plus near ultraviolet light damage in bacteriophages T3 and T4. Photochem Photobiol 33:73–78

    Google Scholar 

  • Thompson LH, Rubin JS, Cleaver JE, Whitmore GF, Brookman K (1980) A screening method for isolating DNA repair-deficient mutant of CHO cells. Somatic Cell Genet 6:391–405

    Google Scholar 

  • Venturini S, Tamaro M, Monti-Bragadin C, Bordin F, Bacchichetti F, Carlassare F (1980) Comparative mutagenicity of linear and angular furocoumarins in Escherichia coli strains deficient in known repair functions. Chem Biol Interact 30:203–207

    Google Scholar 

Download references

Author information

Author notes

  1. T. Saeki

    Present address: Division of Genetics, National Institute of Radiological Sciences, 9-4-1 Chome, Anagawa, Chiba-shi, Japan

Authors and Affiliations

  1. Institut Curie-Biologie, Centre Universitaire, Bâtiment 110, F-91405, Orsay, France

    T. Saeki, C. Cassier & E. Moustacchi

Authors
  1. T. Saeki
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. Cassier
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. Moustacchi
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by W. Gajewski

Rights and permissions

Reprints and permissions

About this article

Cite this article

Saeki, T., Cassier, C. & Moustacchi, E. Induction in Saccharomyces cerevisiae of mitotic recombination by mono and bifunctional agents: Comparison of the pso 2-1 and rad52 repair deficient mutants to the wild-type. Mol Gen Genet 190, 255–264 (1983). https://doi.org/10.1007/BF00330648

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00330648

Keywords

Search

Navigation