Skip to main content
SpringerLink
Log in

Repair and mutation following UV damage in heterokaryons of Neurospora

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

UV damage in a repair-deficient strain has been repaired when a repair-proficient strain has joined it in a heterokaryon. By substituting various repair-deficient mutants for the two components of this system, it has been shown that the rescue is accomplished by means of the excision repair system for pyrimidine dimers. Mutation has been monitored in the rescued nuclei and found to occur with a low frequency. When the rescuing component is replaced by a strain which lacks the excision repair system, there is a low frequency of rescue and the rescued nuclei have a high incidence of mutation. It is concluded that excision repair is relatively mutation-free, but in its absence another repair system is called upon which produces mutations.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Baluch J, Chase JW, Sussman R (1980) Synthesis of recA protein and induction of bacteriophage lambda in single-strand deoxyribonucleic acid-binding protein mutants of Escherichia coli. J Bacteriol 144:489–498

    Google Scholar 

  • Fraser MJ, Chow TYK, Kafer E (1980) Nucleases and their control in wild-type and nuh mutants of Neurospora. In: Generoso M, Shelby M, deSerres F (eds) DNA repair and mutagenesis in eukaryotes. Plenum, New York, pp 63–74

    Google Scholar 

  • Kenyon CJ, Walker GC (1980) DNA-damaging agents stimulate gene expression at specific loci in Escherichia coli. Proc Natl Acad Sci USA 77:2819–2823

    Google Scholar 

  • Schroeder AL (1975) Genetic control of radiation sensitivity and DNA repair in Neurospora. In: Hanawalt PC, Setlow RB (eds) Molecular mechanisms for repair of DNA, part B. Plenum, New York, pp 567–576

    Google Scholar 

  • Shelby MD, deSerres FJ, Stine GJ (1975) Ultraviolet inactivation of conidia from heterokaryons of Neurospora crassa containing UV-sensitive mutations. Mutat Res 27:45–58

    Google Scholar 

  • Shelby MD, Stine GJ, deSerres FJ (1975) Gamma-ray inactivation of conidia from heterokaryons of Neurospora crassa containing UV-sensitive mutations. Mutat Res 28:147–154

    Google Scholar 

  • Stadler D, Crane E (1979) Analysis of lethal events induced by ultraviolet in a heterokaryon of Neurospora. Mol Gen Genet 171:59–68

    Google Scholar 

  • Stadler D, Moyer R (1981) Induced repair of genetic damage in Neurospora. Genetics 98:763–774

    Google Scholar 

  • Stadler DR, Smith DA (1968) A new mutation in Neurospora for sensitivity to ultraviolet. Canad J Genet Cytol 10:916–919

    Google Scholar 

  • Weigle JJ (1953) Induction of mutations in a bacterial virus. Proc Natl Acad Sci USA 39:628–636

    Google Scholar 

  • Worthy TE, Epler JL (1973) Biochemical basis of radiation sensitivity in mutants of Neurospora crassa. Mutat Res 19:167–173

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Genetics SK-50, University of Washington, 98195, Seattle, Washington, USA

    David R. Stadler

Authors
  1. David R. Stadler
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by Ch. Auerbach

Rights and permissions

Reprints and permissions

About this article

Cite this article

Stadler, D.R. Repair and mutation following UV damage in heterokaryons of Neurospora . Mol Gen Genet 190, 227–232 (1983). https://doi.org/10.1007/BF00330644

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00330644

Keywords

Search

Navigation