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Localization of satellite DNAs in the chromosomes of the guinea pig

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Abstract

The in situ hybridization method has been used to investigate the localization of each of the three satellite DNAs present in the genome of the guinea pig. Purified fractions of the satellite DNAs were utilized as templates for synthesis of 3H-labeled complementary RNA (cRNA) by E. coli RNA polymerase, then each cRNA was hybridized to metaphase spreads of embryonic guinea pig cells. The cRNAs of all three satellite DNAs hybridized predominantly to the centromeric region of the chromosomes. The cRNAs of satellite DNAs II and III hybridized to all chromosomes except the Y chromosome. The cRNA of satellite DNA I did not hybridize to the Y chromosome nor to two pairs of small acrocentric chromosomes. Satellite II cRNA hybridized to the telomeric region of chromosomes 3 and 4.

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Authors and Affiliations

  1. Institut du Cancer de Montréal, Centre Hospitalier Notre-Dame, 1560 est, rue Sherbrooke, H2L 4M1, Montréal, Québec, Canada

    N. Duhamel-Maestracci & R. Simard

  2. Department of Biochemistry, McGill University, 3655 Drummond Street, H3G 1Y6, Montreal, Quebec, Canada

    K. Harbers & J. H. Spencer

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  1. N. Duhamel-Maestracci
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  2. R. Simard
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  3. K. Harbers
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  4. J. H. Spencer
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Duhamel-Maestracci, N., Simard, R., Harbers, K. et al. Localization of satellite DNAs in the chromosomes of the guinea pig. Chromosoma 75, 63–74 (1979). https://doi.org/10.1007/BF00330625

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  • DOI: https://doi.org/10.1007/BF00330625

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