Summary
The petumia genome encodes an hsp70 multigene family. Both heat and heavy metals can induce transcription of petunia hsp70 genes. We show that CdCl2 induces at least two family members and that CdCl2 stress, unlike heat stress, interferes with processing of the hsp70 transcripts. We have sequenced a genomic clone corresponding to the sequence of one of the hsp70 cDNAs and found that it contains a 618-bp intron. This intron, which is not efficiently removed during CdCl2 stress, does not appear to encode a novel peptide of any significant length. A preliminary induction of heat shock proteins (hsps) does not protect against the CdCl2 inhibition in Drosophila protects against heat induced inhibition of hsp83 processing (Yost and Lindquist 1986). The mechanism of CdCl2 inhibition of splicing is unclear, however these data support our earlier finding (Rochester et al. 1986) that some stress-inducible plant hsp70 genes, unlike stress-inducible hsp70 genes from other organisms, contain an intron.
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Communicated by R.B. Goldberg
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Winter, J., Wright, R., Duck, N. et al. The inhibition of petunia hsp70 mRNA processing during CdCl2 stress. Mol Gen Genet 211, 315–319 (1988). https://doi.org/10.1007/BF00330609
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DOI: https://doi.org/10.1007/BF00330609