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Zytophotometrische Bestimmung des Histon- und Gesamtproteingehalts von Zellen des lymphatischen Gewebes

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Summary

The various cell types of human lymphatic tissue have been identified on imprints stained after Pappenheim. Thereafter the slides were restained successively with the Feulgen method and with the Fastgreen dye respectively with Naphthol Yellow S. So it was possible to determine the nuclear DNA and histone content and the total proteins of the cell by cytophotometry.

The results give information about the activity of the different cell nuclei and lead to the following classification of the lymph node cells:

  1. 1.

    Regeneratory active cells (germinoblasts, basophilic stem cells, plasmoblasts) show active DNA synthesis, reduction in histone content compared to DNA and very high total protein content of the cell nuclei.

  2. 2.

    Functional active cells (histiomonocytes, reticulum cells) with mostly diploid DNA values, reduced histone content and high total protein content.

  3. 3.

    Low active cells (germinocytes, plasmocytes) with diploid DNA and histone content and only a small increase of total proteins.

  4. 4.

    Inactive cells (lymphocytes) with diploid DNA and histone values and a low total protein content.

Zusammenfassung

An Tupfpräparaten von menschlichen Tonsillen mit chronischer unspezifischer hyperplastischer Entzündung wurden die Zellen des lymphatischen Gewebes auf ihren Gehalt an Histoneiweiß und Gesamtprotein zytophotometrisch untersucht. Den Untersuchungen ging jeweils die DNS-Bestimmung an denselben Zellen voraus.

Nach den erhaltenen DNS-, Histon- und Gesamtproteinwerten der Zellkerne lassen sich vier Gruppen von Zellen unterscheiden:

  1. 1.

    Regeneratorisch aktive Zellen (Germinoblasten, basophile Stammzellen und Plasmoblasten) mit reger DNS-Synthese, vermindertem Histon- und stark erhöhtem Gesamtproteingehalt.

  2. 2.

    Funktioneil aktive Zellen (histiomonocytäre Zellen, große Reticulumzellen) mit weitgehend diploidem DNS-Gehalt, vermindertem Histon- und mäßig stark erhöhtem Gesamteiweißgehalt.

  3. 3.

    Gering aktive Zellen (Germinocyten, Plasmazellen) mit diploidem DNS- und Histongehalt und gering erhöhtem Gesamtproteingehalt.

  4. 4.

    Inaktive Zellen (Lymphocyten) mit diploidem DNS- und Histongehalt und niedrigen Gesamtproteinwerten.

Der Gesamtproteingehalt und das Verhältnis der Histonproteine zur DNS werden im Zusammenhang mit der genetischen Aktivität der Zellkerne diskutiert.

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Authors and Affiliations

  1. Pathologisches Institut der Universität Kiel, Deutschland

    W. Queisser, K. Noeske, W. Sandritter & K. Lennert

  2. Universität Gießen, Deutschland

    W. Queisser, K. Noeske, W. Sandritter & K. Lennert

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  1. W. Queisser
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  2. K. Noeske
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  3. W. Sandritter
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  4. K. Lennert
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Queisser, W., Noeske, K., Sandritter, W. et al. Zytophotometrische Bestimmung des Histon- und Gesamtproteingehalts von Zellen des lymphatischen Gewebes. Zeitschrift für Zellforschung 85, 47–61 (1967). https://doi.org/10.1007/BF00330585

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