Abstract
Mitotic chiasmata and other quadriradials (QRs) were studied by Q-banding in mitomycin C-treated and untreated lymphocytes from two sibs with Bloom's syndrome. The frequency of chiasmata was very significantly increased by the mitomycin treatment in cells from both sibs. Chiasmata occurred throughout the chromosomes, but were favored in Q-dark regions, particularly at borders between dark and light regions (Kuhn, 1976). No significant difference was found in the distribution of chiasmata among chromosome regions in treated and untreated material. This differs from the reported action of mitomycin C on cultured lymphocytes of normal persons, where chiasmata are concentrated at secondary constrictions and centromeres. Adjacent counterparts to mitotic chiasmata, and chromatid translocations between non-homologous chromosomes, also occurred in the treated material, but with a much lower frequency than mitotic chiasmata. This again differs from the effects of mitomycin C on lymphocytes of normal persons, where chiasmata account for 20% or less of total QRs.
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Bourgeois, C.A.: Distribution of mitomycin C-induced damage in human chromosomes with special reference to regions of repetitive DNA. Chromosoma (Berl.) 48, 203–211 (1974)
Evans, H.J.: Molecular architecture of human chromosomes. Brit. med. Bull. 29, 196–202 (1973)
German, J.: Cytological evidence for crossing-over in vitro in human lymphoid cells. Science 144, 298–301 (1964)
German, J.: Bloom's syndrome. I. Genetical and clinical observations in the first twenty-seven patients. Amer. J. hum. Genet. 21, 196–227 (1969)
German, J., Crippa, L.P., Bloom, D.: Bloom's syndrome. III. Analysis of the chromosome aberration characteristic of this disorder. Chromosoma (Berl.) 48, 361–366 (1974)
Giannelli, F., Benson, P.F., Pawsey, S.A., Polani, P.E.: Ultraviolet light sensitivity and delayed DNA-chain maturation in Bloom's syndrome fibroblasts. Nature (Lond.) 265, 466–469 (1977)
Hand, R., German, J.: A retarded rate of DNA chain growth in Bloom's syndrome. Proc. nat. Acad. Sci. (Wash.) 72, 758–762 (1975)
Hand, R., German, J.: Bloom's syndrome: DNA replication in cultured fibroblasts and lymphocytes. Hum. Genet. 38, 297–306 (1977)
Huttner, K.M., Ruddle, F.H.: Study of mitomycin C-induced chromosomal exchange. Chromosoma (Berl.) 56, 1–13 (1976)
Iyer, V.N., Szybalski, W.: Mitomycins and porfiromycin: chemical mechanism of activation and cross-linking of DNA. Science 145, 55–58 (1964)
Kuhn, E.M.: Nonrandom localization of mitotic crossing-over in Bloom's syndrome. Genetics 77, s37-s38 (1974)
Kuhn, E.M.: Localization by Q-banding of mitotic chiasmata in cases of Bloom's syndrome. Chromosoma (Berl.) 57, 1–11 (1976)
Morad, M., Jonasson, J., Lindsten, J.: Distribution of mitomycin C-induced breaks of human chromosomes. Hereditas (Lund) 74, 274–281 (1973)
Patau, K., Therman, E.: Mitotic crossing-over in man. Genetics 61, s45-s46 (1969)
Schroeder, T.M., German, J.: Bloom's syndrome and Fanconi's anemia: demonstration of two distinctive patterns of chromosome disruption and rearrangement. Humangenetik 25, 299–309 (1974)
Shaw, M.W., Cohen, M.M.: Chromosome exchanges in human leukocytes induced by mitomycin C. Genetics 51, 181–190 (1965)
Therman, E., Kuhn, E.M.: Cytological demonstration of mitotic crossing-over in man. Cytogenet. Cell Genet. 17, 254–267 (1976)
Vogel, F., Schroeder, T.M.: The internal order of the interphase nucleus. Humangenetik 25, 265–297 (1974)
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This is paper No. 2054 from the Genetics Laboratory, University of Wisconsin, Madison
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Kuhn, E.M. Mitotic chiasmata and other quadriradials in mitomycin C-treated Bloom's syndrome lymphocytes. Chromosoma 66, 287–297 (1978). https://doi.org/10.1007/BF00330557
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DOI: https://doi.org/10.1007/BF00330557