Summary
The granulosa cells of the ovarian follicle of the rat and the domestic fowl have been studied with the light and electron microscope. The nuclei of the granulosa cells were irregular with indentations and large in proportion to the cytoplasm of the cell. The mitochondria had a dense, dark matrix with only few cristac. The Golgi apparatus was moderately developed, located towards the oocyte in a juxtanuclear position. The endoplasmic reticulum was rather sparse. Lipid droplets were only occasionally encountered. Microtubules were regularly observed. The functions of the granulosa cells are discussed. Compared with the steroid-producing cells of the theca interna of the same follicles, the granulosa cells primarily are the nursing cells for the growing oocyte and mainly have the characteristics of protein forming cells.
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Appelgren, L. E.: Sites of steroid hormone formation. Autoradiographic studies using labelled precursors. Acta physiol. scand., Suppl. 301, 1–108 (1967).
Bjersing, L.: On the morphology and endocrine function of granulosa cells in ovarian follices and corpora lutea. Acta endocr. (Kbh.), Suppl. 125, 1–23 (1967).
Björckman, N.: A study of the ultrastructure of the granulosa cells of the rat ovar. Acta anat. (Basel) 51, 125–147 (1962).
Blanchette, E. J.: Ovarian steroid cells. I. Differentiation of the lutein cell from the granulosa follicle cell during the preovulatory stage and under the influence of exogenous gonadotrophins. J. Cell Biol. 31, 501–516 (1966).
—: Ovarian steroid cells. II. The lutein cell. J. Cell Biol. 31, 517–542 (1966).
Dahl, E.: Studies of the fine structure of ovarian interstitial tissue. 2. The ultrastructure of the thecal gland of the domestic fowl. Z. Zellforsch. 109, 195–211 (1970)a.
—: Studies of the fine structure of ovarian interstitial tissue. 3. The innervation of the thecal gland of the domestic fowl. Z. Zellforsch. 109, 212–226 (1970b).
—: Studies of the fine structure of ovarian interstitial tissue. 6. Effects of clomiphene on the thecal gland of the domestic fowl. Z. Zellforsch. 109, 227–244 (1970c).
—: Studies of the fine structure of ovarian interstitial tissue. 1. A comparative study of the fine structure of the ovarian interstitial tissue in the rat and the domestic fowl. J. Anat. (Lond.) 108, 275–290 (1971a)
—: Studies of the fine structure of ovarian interstitial tissue. 4. Effects of steroids on the thecal gland of the domestic fowl. Z. Zellforsch. 113, 111–132 (1971b).
—: Studies of the fine structure of ovarian interstitial tissue. 5. Effects of gonadotropins on the thecal gland of the domestic fowl. Z. Zellforsch. 113, 133–156 (1971c).
—: The effects of steroids on the granulosa cells in the domestic fowl. Z. Zellforsch. 119, 179–187. (1971d).
- The effects of gonadotropins on the granulosa cells of the domestic fowl. Acta Endocrinologia (in press) (1971e).
—: The effects of clomiphene on the granulosa cells of the domestic fowl. Z. Zellforsch. 119, 188–194 (1971f).
Eckstein, P.: The ovary. In: Zuckerman, S. (ed.), p. 311. New York: Academic Press 1962.
Enders, A. C.: Observations on the fine structure of lutein cells. J. Cell Biol. 12, 101–113 (1962).
Falck, B.: Site of production of oestrogen in rat ovary as studied in microtransplants. Acta physiol. scand. 47, Suppl. 163, 1–101 (1959).
Kjaerheim, Å.: Studies of adrenocortical ultrastructure. 1. Aldehyde perfusion fixation of the domestic fowl. Acta anat. (Basel) 74, 424–453 (1969).
Millonig, G.: The advantages of a phosphate buffer for OsO4 solutions in fixation. J. appl. Physiol. 32, 1637 (1961).
Mossmann, H. W., Koering, M. J., Ferry, D., Jr.: Cyclic changes of interstitial gland tissue of the human ovary. Amer. J. Anat. 115, 235–256 (1964).
Reynolds, E. S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963).
Ryan, K. J., Smith, O. W.: Biogenesis of steroid hormones in the human ovary. Recent Progr. Hormone Res. 21, 367–409 (1965).
Ryter, A., Kellenberger, E.: L'inclusion au polyester pour l'ultramicrotomie. J. Ultrastruct. Res. 2, 200–214 (1958).
Westman, A.: Experimentelle Studien über die funktionelle Bedeutung der Theca-internaZellen. Acta obstet, gynec. scand. 8, 290–306 (1929).
—: Untersuchungen über die Abhängigkeit der Funktion des Corpus luteum von den Ovarialfollikeln und über die Bildungsstätte der Hormone im Ovarium. Arch. Gynäk. 158, 476–504 (1934).
Woods, J. E., Domm, L. V.: A histochemical identification of the Androgen-producing cells in the gonads of the domestic fowl and albino rat. Gen. comp. Endocr. 7, 559–570 (1966).
Wyburn, G. H., Baillie, A. H.: In: Horton-Smith, C., and Amoroso, E. C. (ed.), Physiology of the domestic fowl, p. 30. Edinburg: Oliver and Boyd 1966.
Young, W. C.: The mammalian ovary. In: Sex and internal secretion, 3rd. ed., (W. C. Young, ed.), p. 449. Baltimore: Williams and Wilkins Co. 1961.
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Dahl, E. The fine structure of the granulosa cells in the domestic fowl and the rat. Z. Zellforsch. 119, 58–67 (1971). https://doi.org/10.1007/BF00330538
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DOI: https://doi.org/10.1007/BF00330538