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Achlya mitochondrial DNA: gene localization and analysis of inverted repeats

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Summary

Mitochondrial DNA from four strains of the oomycete Achlya has been compared and nine gene loci mapped, including that of the ribosomal protein gene, var1. Examination of the restriction enzyme site maps showed the presence of four insertions relative to a map common to all four strains. All the insertions were found in close proximity to genic regions. The four strains also cotained the inverted repeat first observed in A. ambisexualis (Hudspeth et al. 1983), allowing an examination by analysis of retained restriction sites of the evolutionary stability of repeated DNA sequences relative to single copy sequences. Although the inverted repeat is significantly more stable than single copy sequences, more detailed analysis indicated that this stability is limited to the portion encoding the ribosomal RNA genes. Thus, the apparent evolutionary stability of the repeat does not appear to derive from the inverted repeat structure per se.

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Abbreviations

ATPase 6, 9:

genes for ATPase subunits 6 and 9

COI, II, III:

genes for cytochrome oxidase subunits 1, 2, and 3

COB:

gene for apocytochrome b

L-, S-RNA:

genes for the mitochondrial large and small ribosomal RNAs

mtDNA:

mitochondrial DNA

var1 :

gene for the S. cerevisiae mitochondrially, encoded ribosomal protein

m.u.:

map units

bp:

base pairs

kb:

kilobase pairs

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Author notes

  1. Deborah S. Shumard & Michael E. S. Hudspeth

    Present address: Plant Molecular Biology Center, Department of Biological Sciences, Northern Illinois University, 60115-2861, DeKalb, IL, USA

  2. Lawrence I. Grossman

    Present address: , Science 1333 H Street N.W., 20005, Washington DC, USA

Authors and Affiliations

  1. Department of Cellular and Molecular Biology, Division of Biological Sciences, The University of Michigan, 48109, Ann Arbor, MI, USA

    Deborah S. Shumard, Lawrence I. Grossman & Michael E. S. Hudspeth

Authors
  1. Deborah S. Shumard
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  2. Lawrence I. Grossman
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  3. Michael E. S. Hudspeth
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Communicated by R. B. Goldberg

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Shumard, D.S., Grossman, L.I. & Hudspeth, M.E.S. Achlya mitochondrial DNA: gene localization and analysis of inverted repeats. Mol Gen Genet 202, 16–23 (1986). https://doi.org/10.1007/BF00330510

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  • DOI: https://doi.org/10.1007/BF00330510

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