Summary
Plasmid pIF132 containing two direct repeats of the mel (melanin) sequence was used to monitor intraplasmid recombination. Five mutants of Streptomyces lividans TK64 deficient in intraplasmid recombination were isolated. Four contained additional defects in aerial mycelium formation, pigmentation, and nutrient requirements; among these two showed extensive amplification of chromosomal sequences. Mutant JT46 had no pleiotropic defects but had the most severe blockage in recombination. Only one of the mutants was slightly more sensitive to UV and two were slightly more sensitive to mitomycin C. Plasmid pWCL1 (containing pIJ702, pUC12, and HBVsAg sequences; Lee et al. 1986) could not stably replicate in TK64 without spontaneous deletions. In contrast the mutant JT46 maintained the integrity of pWCL1 much more stably.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Albertini MA, Hofer M, Calos MP, Miller JH (1982) On the formation of spontaneous deletions: The importance of short sequence homologies in the generation of large deletions. Cell 29:319–328
Altenbuchner J, Cullum J (1984) DNA amplification and an unstable arginine gene in Streptomyces lividans 66. Mol Gen Genet 195:134–138
Baltz RH, Stonesifer J (1985) Mutagenic and error-free DNA repair in Streptomyces. Mol Gen Genet 200:351–355
Benigni R, Antonov RP, Carere A (1975) Estimate of the genome size by renaturation studies in Streptomyces. Appl Microbiol 30:324–326
Berman V, Filpula D, Herber W, Bibb M, Katz E (1985) The nucleotide sequence of the tyrosinase gene from Streptomyces antibioticus and characterization of the gene product. Gene 37:101–110
Chater KF, Hopwood DA, Kieser T, Thompson CJ (1982) Gene cloning in Streptomyces. Curr Top Microbiol 96:69–95
Chen CW, Thomas Jr CA (1983) A rapid two-dimensional fractionation of DNA restriction fragments. Anal Biochem 131:397–403
Chen CW, Tasi JF-Y, Chuang S-E (1987) Intraplasmid recombination in Streptomyces lividans 66. Mol Gen Genet 207:441–445
Chuang S-E (1984) Expression of cloned melanin gene in Streptomyces. M. Sc Thesis, National Yang-ming Medical College, Institute of Microbiology and Immunology
Clark AJ (1973) Recombination-deficient mutants of E. coli and other bacteria. Annu Rev Genet 7:67–86
Clark AJ, Margulies AD (1965) Isolation and characterization of recombination-deficient mutants of Escherichia coli K12. Proc Natl Acad Sci USA 53:451–459
Cohen A, Laban A (1983) Plasmidic recombination in Escherichia coli K-12: The role of recF gene function. Mol Gen Genet 189:471–474
Delié V, Hopwood DA, Friend EJ (1970) Mutagenesis by N-methyl-N′-nitro-N-nitrosoguanidine (NTG) in Streptomyces coelicolor. Mutat Res 9:167–182
Dressler D, Potter H (1982) Molecular mechanisms in genetic recombination. Annu Rev Biochem 51:727–761
Enquist LW, Bradley SG (1971) Characterization of deoxyribonucleic acid from Streptomyces venezuelae spores. Dev Ind Microbiol 12:225–236
Fishel RA, Kolodner R (1984) Escherichia coli strains containing mutations in the structural gene for topoisomerase I are recombination deficient. J Bacteriol 160:1168–1170
Harold RJ, Hopwood DA (1970) Ultraviolet-sensitive mutants of Streptomyces coelicolor. Mutat Res 10:427–438
Hopwood DA, Chater KF (1984) Streptomyces. In: Ball C (ed) Genetics and Breeding of industrial Microorganisms, CRC Press, Boca Raton, Florida, USA, pp 7–42
Hopwood DA, Lydiate DJ, Malpartida F, Wright HW (1985) Conjugative sex plasmids of Streptomyces. In: Helinski DR, Cohen SN, Clewell DB, Jackson DA, Hollaender A, Hager L, Kaplan S, Konisky J, Wilson CM (eds) Plasmids in Bacteria, Plenum Press, New York, USA, pp 615–634
James AA, Morrison PT, Kolodner R (1982) Genetic recombination of bacterial plasmid DNA — Analysis of the effect of recombination-deficient mutations on plasmid recombination. J Mol Biol 160:411–430
Jones IM, Primorose SB, Ehrlich SD (1982) Recombination between short direct repeats in a RecA host. Mol Gen Genet 188:486–489
Katz E, Thompson CJ, Hopwood DA (1983) Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans. J Gen Microbiol 129:2703–2714
Kieser T (1984) Factors affecting the isolation of ccc DNA from Streptomyces lividans and Escherichia coli. Plasmid 12:19–36
Kolodner R, Fishel RA, Howard M (1985) Genetic recombination of bacterial plasmid DNA: Effect of recF pathway mutations on plasmid recombination in Escherichia coli. J Bacteriol 163:1060–1066
Lee Y-HW, Tzechen Z-Y, Wang S-C, Chen W-L, Chen CW (1986) Structural stability of heterologous genes cloned in Streptomyces plasmid pIJ702. Biochem Biophys Res Commun 140:372–378
Lo SJ, Lee YHW, Chiou JS, Ting LP, Liu WT, Choo KB (1985) Characterization of restriction endonuclease maps of Hepatitis B viral DNAs. Biochem Biophys Res Commun 129:797–803
Low B (1968) Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12. Proc Natl Acad Sci USA 60:160–165
McCorkle GM, Altman S (1982) Large deletion mutants of Escherichia coli tRNA1 Tyr. J Mol Biol 155:83–103
Messing J (1983) New M13 vectors for cloning. Methods Enzymol 101:20–78
Miller JH (1972) Experiments in Molecular Genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Nakano MM, Ogawara H, Sekiya T (1984) Recombination between short direct repeats in Streptomyces lavendulae plasmid DNA. J Bacteriol 157:658–660
O'Connell MP (1984) Genetic transfer in prokaryotes: Transformation, transduction, and conjugation. In: Pühler A, Timmis KN (eds) Advanced Molecular Genetics, Springer-Verlag, Berlin heidelberg, pp 2–13
Schrempf H (1982) Plasmid loss and changes within the chromosomal DNA of Streptomyces reticuli. J Bacteriol 151:701–707
Stonesifer J, Baltz RH (1985) Mutagenic DNA repair in Streptomyces. Proc Natl Acad Sci USA 82:1180–1183
Vieira J, Messing J (1982) The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268
Walker GC (1985) Inducible DNA repair systems. Annu Rev Biochem 54:425–457
Author information
Authors and Affiliations
Additional information
Communicated by N.D.F. Grindley
Rights and permissions
About this article
Cite this article
Tsai, J.F.Y., Chen, C.W. Isolation and characterization of Streptomyces lividans mutants deficient in intraplasmid recombination. Mole Gen Genet 208, 211–218 (1987). https://doi.org/10.1007/BF00330444
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00330444