Summary
High molecular weight genomic DNA isolated from the archaebacterium Methanococcus voltae by alkaline-SDS lysis was not effectively digested with the restriction enzyme Sau3AI, which recognizes the base sequence GATC. Mc. voltae DNA was also resistant to digestion by MboI and BamHI which recognize sites containing the same GATC sequence. Examination of a Mc. voltae genomic library prepared in Escherichia coli JM83 with a pUC vector revealed that the 5–10 kb inserts were still resistant to Sau3AI digestion, indicating a likely lack of the GATC sequence in Mc. voltae DNA.
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Jarrell, K.F., Julseth, C., Pearson, B. et al. Paucity of the Sau3AI recognition sequence (GATC) in the genome of Methanococcus voltae . Mole Gen Genet 208, 191–194 (1987). https://doi.org/10.1007/BF00330441
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DOI: https://doi.org/10.1007/BF00330441