Summary
Three constitutive DNase I hypersensitive sites were mapped between the transcription start site (+1) and-300 bp in the chromatin of the maize alcohol dehydrogenase-2 (Adh2) gene. The first site maps at-40 to-70, the second at-150 to-180, and the third at-250 to-285. These sites are present in the chromatin of nuclei from both roots and suspension culture cells. All three hypersensitive sites appear in hypoxic (induced) as well as aerobic (uninduced) states of the gene. However, on induction the sites appear more pronounced and one of these sites (mapping at-40 to-70) extends through the TATA box to the transcription start site. Purified genomic DNA as well as plasmid DNA carrying the same cloned fragment of the Adh2 promoter, show intense hypersensitivity at two sites, between-250 to-360 and-410 to-500. The site between-250 to-360 is present in chromatin but partially protected. The site between-410 to-500 is completely protected in chromatin.
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References
An G, Ebert P, Yi B-Y, Choi C-H (1986) Both TATA box and upstream regions are required for the nopaline synthase promoter activity in transformed tobacco cells. Mol Gen Genet 203:245–250
Ashburner M, Bonner J (1979) The induction of gene activity in Drosophila by heat shock. Cell 17:241–254
Breathnack R, Chambon P (1981) Organization and expression of eukaryotic split genes coding for proteins. Annu Rev Biochem 50:349–383
Bryan P, Olah J, Birnstiel M (1983) Major changes in the 5′ and 3′ chromatin structure of sea urchin histone genes accompany their activation and inactivation in development. Cell 33:843–848
Church G, Gilbert W (1984) Genomic sequencing. Proc Natl Acad Sci USA 81:1991–1995
Costlow N, Lis J (1984) High-resolution mapping of DNase-I hypersensitive sites of Drosophila heat shock genes in Drosophila melanogaster and Saccharomyces cerevisiae. Mol Cell Biol 4:1853–1863
Dellaporta S, Wood J, Hicks J (1983) A plant DNA minipreparation, Version II. Plant Mol Biol Rep 1:19–23
Dennis E, Sachs M, Gerlach W, Finnegan W, Peacock W (1985) Molecular analysis of the alcohol dehydrogenase 2(Adh2) gene of maize. Nucleic Acids Res 13:727–743
Eissenberg J, Cartwright I, Thomas G, Elgin S (1985) Selected topics in chromatin structure. Annu Rev Genet 19:485–536
Elgin S (1981) DNase-I hypersensitive sites of chromatin. Cell 27:413–415
Freeling M (1973) Simultaneous induction by anaerobiosis or 2,4-D of multiple enzymes specified by two unlinked genes: Differential Adh1-Adh2 expression in maize. Mol Gen Genet 127:215–227
Freeling M (1974) Dimerization of multiple maize ADHs studied in in vivo and in vitro. Biochem Genet 12:407–417
Freeling M, Schwartz D (1973) Genetic relationships between the multiple alcohol dehydrogenases of maize. Biochem Genet 8:27–36
Fritton H, Igo-Kemenes T, Howock J, Strech-Jurk U, Theisen M, Sippel A (1984) Alternative sets of DNase-I hypersensitive sites characterize the various functional states of the chicken lysozyme gene. Nature 311:163–165
Grosveld G, Shewmaker C, Jat P, Flavell R (1981) Localization of DNA sequences necessary for transcription of the rabbit β-globin gene in vitro. Cell 25:215–226
Grosveld G, de Boer E, Shewmaker C, Favell R (1982) DNA sequences necessary for transcription of the rabbit β-globin gene in vivo. Nature 295:120–126
Jongstra J, Reudelhuber T, Oudet P, Benoist C, Chae C-B, Jeltsch J, Mathis D, Chambon P (1984) Induction of altered chromatin structures by simian virus 40 enhancer and promoter elements. Nature 307:708–714
Kaye J, Pratt-Kaye S, Bellard M, Dretzen G, Bellard F, Chambon P (1986) Steroid hormone dependence of four DNase-I hypersensitive regions located within the 7000-bp 5′-flanking segment of the ovalbumin gene. EMBO J 5:277–285
Keene M, Corces V, Lowenhaupt K, Elgin E (1981) DNase-I hypersensitive sites in Drosophila chromatin occur at the 5′ ends of the regions of transcription. Proc Natl Acad Sci USA 78:143–146
Lowenhaupt K, Cartwright I, Keene M, Zimmerman J, Elgin S (1983) Chromatin structure in pre- and postblastula embryos of Drosophila. Dev Biol 99:194–201
Maniatis T, Fritsch E, Sambrook J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory. Cold Spring Harbor, New York
McKnight S, Kingsbury R (1982) Transcriptional control signals of a eukaryotic protein coding gene. Science 217:316–324
Nevins J (1983) The pathway of eukaryotic mRNA formation. Annu Rev Biochem 52:441–466
Paul A-L, Vasil V, Vasil IK, Ferl R (1987) Constitutive and anaerobically induced DNase-I hypersensitive sites in the 5′ region of the maize Adh1 gene. Proc Natl Acad Sci USA, 84:799–803
Rivin C, Zimmerman E, Walbot V (1982) Isolation of DNA and DNA recombinants from maize. In: Sheridan WF (ed) Maize for biological research. University of North Dakota Press
Senear A, Palmiter R (1982) Expression of the mouse metallothionein-I gene alters the nuclease hypersensitivity of its 5′ regulatory region. Cold Spring Harbor Symp Quant Biol 47:539–547
Shermoen A, Beckendorf S (1982) A complex of interacting DNase-I hypersensitive sites near the Drosophila glue protein gene, Sgs 4. Cell 29:601–607
Sledziewski A, Young E (1982) Chromatin conformational changes accompany transcriptional activation of a glucose-repressed gene in Saccharomyces cervisiae. Proc Natl Acad Sci USA 79:253–256
Thomas G, Siegfried E, Elgin S (1985) DNase-I hypersensitive sites: A structural feature of chromatin associated with gene expression. In: Reeck G, Goodwin G, Puigdomenech S: Chromosomal proteins and gene expression. Plenum Press, New York
Vasil V, Vasil IK (1986) Plant regeneration from friable embryogenic callus and cell suspension cultures of Zea mays L. J Plant Physiol 124:399–408
Weintraub H, Groudine M (1976) Chromosome subunits in active genes have an altered conformation. Science 193:848–856
Weintraub H, Beug H, Groudine M, Graf T (1982) Temperature sensitive changes in the structure of globin chromatin in lines of red cell precursors transformed by a ts-AEV virus. Cell 38:931–940
Wu C (1980) The 5′ ends of Drosophila heat shock genes are hypersensitive to DNase-I. Nature 286:854–860
Wu C, Wong Y-C, Elgin S (1979) The chromatin structure of specific genes: II. Disruption of chromatin structure during gene activity. Cell 16:807–814
Zaret K, Yamamoto K (1984) Reversible and persistent changes in chromatin structure accompany activation of a glucocorticoid-dependent enhancer element. Cell 38:29–38
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Ashraf, M., Vasil, V., Vasil, I.K. et al. Chromatin structure at the 5′ promoter region of the maize Adh2 gene and its role in gene regulation. Mole Gen Genet 208, 185–190 (1987). https://doi.org/10.1007/BF00330440
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DOI: https://doi.org/10.1007/BF00330440