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Characterization of a null-allele for the Gy 4 glycinin gene from soybean

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Summary

A null allele for the Gy 4 glycinin gene from the cultivar Raiden was sequenced and compared with a functional Gy 4 gene from another cultivar. The results showed that the null phenotype probably resulted from a point mutation that changed the translation initiation codon from ATG to ATA. Transcripts of the mutant gene were detected in total RNA from seed, but the mRNAs did not become associated with polysomes as did functional Gy 4 meassage in the control cultivar. This was probably due to premature dissociation of mutant gy 4 mRNA from ribosomes due to nonsense codons during translation of an incorrect reading frame.

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Abbreviations

bp:

base pairs

kb:

kilobase pairs

DNase I:

deoxyribonuclease I

EDTA:

ethylenediaminetetraacetic acid

RNAase TI:

ribonuclease TI

SDS:

sodium dodecyl sulfate

TLE:

10 mM Tris, 0.1 mM EDTA

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Communicated by R.B. Goldberg

Cooperative research between USDA/ARS and the Indiana Agricultural Experiment Station. This is Journal Paper No. 10,884 from the Purdue Agricultural Experiment Station. Mention of a trademark or proprietary product does not constitute a guarantee or warranty of the product by USDA/ARS on Purdue University

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Scallon, B.J., Dickinson, C.D. & Nielsen, N.C. Characterization of a null-allele for the Gy 4 glycinin gene from soybean. Mole Gen Genet 208, 107–113 (1987). https://doi.org/10.1007/BF00330430

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  • DOI: https://doi.org/10.1007/BF00330430

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