Summary
The rfb gene cluster of Salmonella typhimurium encodes the enzymes required for the biosynthesis of the O-Antigen. A part of it has been cloned in plasmid vectors pBR322 and pUC9 using an adjacent, previously cloned, part of the his operon (Barnes 1981) as a molecular probe for the first clone. A detailed restriction enzyme map of 7.57 kb of rfb DNA is presented and the approximate locations of two of the genes, rfbK and rfbM have been defined.
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Brahmbhatt, H.N., Quigley, N.B. & Reeves, P.R. Cloning part of the region encoding biosynthetic enzymes for surface antigen (O-antigen) of Salmonella typhimurium . Mol Gen Genet 203, 172–176 (1986). https://doi.org/10.1007/BF00330399
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DOI: https://doi.org/10.1007/BF00330399