Summary
The gene for the apoprotein of cytochrome b-559 has been located in wheat chloroplast DNA by coupled transcription-translation of fragments of chloroplast DNA in a cell-free system from Escherichia coli and by nucleotide sequence analysis. The gene is located 1.5 kbp downstream from the gene for cytochrome f and is transcribed in the opposite direction. Nucleotide sequence determination revealed an open reading frame coding for a hydrophobic protein of 83 amino acid residues (M r 9434). The deduced amino acid sequence of the first 27 codons corresponds exactly to the determined N-terminal amino acid sequence of spinach cytochrome b-559. Another open reading frame of 39 codons is located 10 bp downstream from the gene for cytochrome b-559. A putative ribosome-binding site, AGGAGG, for this open reading frame overlaps the translation stop codon, UAG, of the cytochrome b-559 gene. The deduced amino acid sequence from this open reading frame shows similarities to the N-terminal sequence of cytochrome b-559. Both sequences contain a histidine residue located in a putative membrane-spanning region of the polypeptide.
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Communicated by R.G. Herrmann
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Hird, S.M., Willey, D.L., Dyer, T.A. et al. Location and nucleotide sequence of the gene for cytochrome b-559 in wheat chloroplast DNA. Mol Gen Genet 203, 95–100 (1986). https://doi.org/10.1007/BF00330389
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DOI: https://doi.org/10.1007/BF00330389