Summary
Candida pelliculosa var. acetaetherius is a strain of yeast which can utilize cellobiose as the carbon source. From a gene library prepared from this yeast, the β-glucosidase gene has been cloned in a S. cerevisiae host using a chromogenic substrate, 5-bromo-4-chloro-3-indolyl-β-glucoside as an indicator. It was proved by Southern analysis that the DNA fragment carrying the β-glucosidase gene originated from C. pelliculosa. β-Glucosidase produced by S. cerevisiae transformants was secreted into the periplasmic space. In Candida, β-glucosidase was not induced by cellobiose but was derepressed by lowering the concentration of glucose. The regulation of β-glucosidase synthesis in S. cerevisiae carrying the cloned β-glucosidase was not clear compared with that in Candida, however, the enzyme activity in low glucose medium (0.05%) was reproducibly higher than in high glucose medium (2%). We have found the sequence that controls the expression of the β-glucosidase gene negatively in S. cerevisiae.
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Communicated by W. Gajewski
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Kohchi, C., Toh-e, A. Cloning of Candida pelliculosa β-glucosidase gene and its expression in Saccharomyces cerevisiae . Mol Gen Genet 203, 89–94 (1986). https://doi.org/10.1007/BF00330388
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DOI: https://doi.org/10.1007/BF00330388