Summary
An experimental analysis of the fate of transpson Tn10 after excision from a proA:: Tn10 site localized on the plasmid F′ leads to the conclusions:
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1.
The precise excision is a progressive process. Its probability is estimated per time unit.
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2.
An excised Tn10 is always integrated into a different genetic locus.
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3.
The kinetics of postexcision transposition are sometimes very slow. The excised transposon is inherited in one cell line in spite of cell multiplication.
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4.
The processes of excision and secondary insertion have no absolute requirement for the recA + genotype but they are strongly enhanced in recA + cells.
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5.
The kinetics of postexcision transposition are strongly dependent on the genetic site from which the transposon was excised.
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6.
The probability of postexcision transposition is fully determined by the probability of excision and depends on the genotype of the host and many other factors.
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References
Arber W, Hümbelin M, Caspers P, Reif HJ, Iida S, Meyer J (1981) Spontaneous mutations in the Escherichia coli prophage P1 and IS-mediated processes. Cold Spring Harbor Symp Quant Biol 45:38–39
Bachmann BJ, Low KB (1980) Linkage map of Escherichia coli K-12. Microb Rev 44:1–56
Berg DE (1977) Insersion and excision of the transposable kanamycin resistance determinat Tn5. In: Bukhari A, Shapiro JA, Adhya S (eds) DNA insertion elements plasmids and episomes. Cold Spring Harbor Laboratory, Cold Spring Harbor Press, pp 205–212
Berg DE, Johnsrud L, McDivitt L, Ramabhadron R, Hirschel SJ (1982) Inverted repeates of Tn5 are transposable elements. Proc Natl Acad Sci USA 79:2632–2635
Botstein D, Kleckner N (1977) Translocation and illegitimate recombination by the tetracycline resistance element Tn10. See Berg (1977) pp 185–204
Bresler SE, Krivonogov SV, Lanzov VA (1979) Genetic determination of the donor properties in Escherichia coli K-12. Phenomena of chromosome mobilization and integrative suppression. Mol Gen Genet 177:177–184
Bresler SE, Goryshin IYu, Lansov VA (1981) The process of general recombination in Escherichia coli K-12. Structure of intermediate products. Mol Gen Genet 183:139–143
Bukhari AI (1981) Models of DNA transposition. Trends Biol Sci 6:56–60
Cornelis G (1980) Transposition of Tn951 (Tn lac) and cointegrate formation are thermosensitive processes. J Gen Microbiol 117:367–374
Egner C, Berg DE (1981) Excision of Tn5 is dependent on the inverted repeats but not on the transposase function of Tn5. Proc Natl Acad Sci USA 78:453–463
Foster TJ, Davis MA, Roberts DE, Takeshita K, Kleckner N (1981a) Genetic organization of transposon Tn10. Cell 23:201–213
Foster TJ, Lundblad V, Hanly-Way S, Halling SM, Kleckner N (1981b) Three Tn10-associated excision events: relationship to transposition and role of direct and indirect repeats. Cell 23:215–217
Hirschel BJ, Berg DE (1982) A derivative of Tn5 with direct terminal repeats can transpose. J Mol Biol 155:105–120
Hopkins, JD, Clements MB, Liang TY, Isberg RR, Syvanen M (1980) Recombination genes on the Escherichia coli sex factor specific for transposable elements. Proc Natl Acad Sci USA 77:2814–2818
Kleckner N (1981) Transposable elements in prokaryotes. Ann Rev Genet 15:341–404
Miller HI, Friedman DI (1980) An Escherichia coli gene product required for lambda aite specific recombination. Cell 20:711–720
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Communicated by D.M. Goldfarb
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Bresler, S.E., Tamm, S.E., Goryshin, I.Y. et al. Postexcision transposition of the transposon Tn10 in Escherichia coli K12. Mol Gen Genet 190, 139–142 (1983). https://doi.org/10.1007/BF00330336
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DOI: https://doi.org/10.1007/BF00330336