Summary
A new type of Escherichia coli K12 phoB mutant was isolated as 5-fluorouracil-plus-adenosine-resistant derivatives of a upp phoS, T strain. Such phoB mutants (called type III) differ from previously described phoB mutants (types I and II) in the synthesis pattern of phosphate-regulated periplasmic proteins P4a and 30.5 K, sensitivity to toxic compounds, and several other phenotypic characters. The analysis of isogenic strains carrying phoB mutations (type I, II or III) showed that; the phoB gene product exerted (i) a positive control over the synthesis of periplasmic proteins 30.5 K, 11.5 K, and 9 K, inner membrane proteins 32 K and 17.5 K, and outer membrane protein Tsx, (ii) and a direct or indirect negative control over the synthesis of a 20 K phosphate-inducible periplasmic protein.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Amemura M, Shinagawa H, Makino K, Otsuji N, Nakata A (1982) Cloning of and complementation tests with alkaline phosphatase regulatory genes (phoS and phoT) of Escherichia coli. J Bacteriol 152:692–701
Argast M, Boos W (1980) Co-regulation in Escherichia coli of a novel transport system for sn-glycerol-3-phosphate and outer membrane protein Ic (e, E) with alkaline phosphatase and phosphate-binding protein. J Bacteriol 143:142–150
Bachmann BJ, Low KJ (1980) Linkage map of Escherichia coli K-12. Edition 6. Microbiol Rev 44:1–56
Bracha M, Yagil E (1969) Genetic mapping of the phoR regulator gene of alkaline phosphatase in Escherichia coli K-12. J Gen Microbiol 59:77–81
Brickman E, Beckwith J (1975) Analysis of the regulation of Escherichia coli alkaline phosphatase synthesis using deletions and ϕ80 transducing phages. J Mol Biol 96:307–316
Echols H, Garen S, Torriani AM (1961) Genetic control of repression of alkaline phosphatase in E. coli. J Mol Biol 3:425–438
Garen A, Echols H (1962) Genetic control of induction of alkaline phosphatase synthesis in E. coli. Proc Natl Acad Sci USA 48:1398–1402
Hantke K (1976) Phage T6-colicin K receptor and nucleoside transport in Escherichia coli. FEBS Lett 70:109–112
Heppel LA (1971) The concept of periplasmic enzymes. In: Rothfield LI (ed) Structural and function of biological membranes. Academic Press Inc, New York, pp 223–247
Heyde M, Portalier R (1982) New pleiotropic alkaline phosphatase-negative mutants of Escherichia coli K-12. J Bacteriol 151:529–533
Kreuzer K, Pratt C, Torriani AM (1975) Genetic analysis of regulatory mutants of alkaline phosphatase of E. coli. Genetics 81:459–468
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680–685
Lazzaroni JC, Portalier RC (1981) Genetic and biochemical characterization of periplasmic-leaky mutants of Escherichia coli K-12. J Bacteriol 145:1351–1358
Mallick U, Herrlich P (1979) Regulation of synthesis of a major outer membrane protein: Cyclic AMP represses Escherichia coli protein III synthesis. Proc Natl Acad Sci USA 76:5520–5523
Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Morris H, Schlesinger MJ, Bracha M, Yagil E (1974) Pleiotropic effects of mutations involved in the regulation of Escherichia coli K-12 alkaline phosphatase. J Bacteriol 119:583–592
Nesmeyanova MA, Tsfasman IM, Kulaev IS (1981) Changes of the membrane protein composition in the mutants on regulatory genes of Escherichia coli alkaline phosphatase. FEBS Lett 124:210–214
Neu HC (1968) The 5′-nucleotidase and cyclic phosphodiesterases (3′-nucleotidases) of Enterobacteriacae. J Bacteriol 95:1732–1737
Tommassen J, de Geus P, Lugtenberg B (1982) Regulation of the pho regulon of Escherichia coli K-12. Cloning of the regulatory genes phoB and phoR and identification of their gene products. J Mol Biol 157:265–274
Tommassen J, Lugtenberg B (1980) Outer membrane protein e of Escherichia coli K-12 is co-regulated with alkaline phosphatase. J Bacteriol 143:151–157
Torriani AM (1967) Alkaline phosphatase from Escherichia coli. In: Cantoni GL, Davies DR (eds) Procedures in nucleic acid research. Harper and Row Publishers Inc, NY, pp 224–235
Wanner BL, Latterell P (1980) Mutants affected in alkaline phosphatase expression: Evidence for multiple positive regulators of the phosphate regulon in Escherichia coli K-12. Genetics 96:353–366
Willsky GR, Malamy MH (1976) Control of the synthesis of alkaline phosphatase and the phosphate-binding protein in Escherichia coli. J Bacteriol 127:595–609
Worcel A, Burgi E (1974) Properties of a membrane-attached form of the folded chromosome of Escherichia coli. J Mol Biol 82:91–105
Yagil E, Bracha M, Lifshitz Y (1975) The regulatory nature of the phoB gene for alkaline phosphatase synthesis in Escherichia coli. Mol Gen Genet 137:11–16
Author information
Authors and Affiliations
Additional information
Communicated by A. Böck
Rights and permissions
About this article
Cite this article
Heyde, M., Portalier, R. Isolation and characterization of a new type of Escherichia coli K12 phoB mutants. Mol Gen Genet 190, 122–127 (1983). https://doi.org/10.1007/BF00330333
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00330333