Skip to main content
SpringerLink
Log in

The products of gene A of the related phages Mu and D108 differ in their specificities

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

By recombination between different mutants of mutator phages Mu and D108, we isolated a set of viable hybrids. The structure of the hybrids was analyzed by digestion with different restriction enzymes. Genetic studies show that hybrids which carry the left end of the Mu genome complement a mini-Mu deleted from within the A gene as well as Mu while hybrids with the left end of the D108 genome or D108 do not. Vice versa, hybrids with the left end of the D108 genome or D108, but not hybrids with the left end of the Mu genome or Mu complement a mini-D108 deleted from within the A gene. The nucleotide sequence of the A gene of Mu and its equivalent on D108 are mainly similar except on their left end. These observations demonstrate that the two pA products, although only partially different, have different specificities.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Adams MH (1959) Bacteriophages. Interscience Publishers, New York

    Google Scholar 

  • Allet B, Bukhari AI (1975) Analysis of Mu and Lamda-Mu hybrid DNAs by specific endonucleases. J Mol Biol 92:529–540

    Google Scholar 

  • Appleyard RK (1954) Segregation of lambda lysogenicity during bacterial recombination in E. coli K12. Genetics 39:429–439

    Google Scholar 

  • Bachmann BJ, Low KB (1980) Linkage map of Escherichia coli K12, Edition 6. Microbiol Rev 44:1–56

    Google Scholar 

  • Bukhari AI, Froshauer S, Botchan M (1976) The ends of bacteriophage Mu DNA. Nature 264:580–583

    Google Scholar 

  • Bukhari AI, Ljungquist E (1977) Bacteriophage Mu: methods for cultivation and use. In: Bukhari AI, Shapiro JA, Adhya S (eds) DNA insertion elements, plasmids and episomes. Cold Spring Harbor Laboratory, NY, pp 749–756

    Google Scholar 

  • Chow LT, Bukhari AI (1977) The invertible DNA segments of coliphage Mu and P1 are identical. Virology 74:242–248

    Google Scholar 

  • Chow LT, Kahmann R, Kamp D (1977) Electron microscopic characterization of DNAs from non-defective deletion mutants of bacteriophage Mu. J Mol Biol 113:591–609

    Google Scholar 

  • Chow LT, Broker T (1978) Adjacent insertion sequences IS2 and IS5 in bacteriophage Mu mutants and IS5 in a lambda darg bacteriophage. J Bacteriol 133:1427–1436

    Google Scholar 

  • Daniell E, Abelson J, Kim JS, Davidson N (1973) Heteroduplex structures of bacteriophage Mu DNA. Virology 51:237–239

    Google Scholar 

  • Daniell E, Kohne DE, Abelson J (1975) Characterization of the inhomogeneous DNA in virions of bacteriophage Mu by DNA reannealing kinetics. J Virol 15:739–743

    Google Scholar 

  • Desmet L, Faelen M, Lefebvre N, Resibois A, Toussaint A, van Gijsegem F (1981) Genetic study of Mu mediated chromosomal rearrangements. Cold Spring Harbor Symp Quant Biol 45:355–363

    Google Scholar 

  • Faelen M, Toussaint A (1973) Isolation of conditional defective mutants of temperate phage Mu-1 and deletion mapping of the Mu-1 prophage. Virology 54:117–124

    Google Scholar 

  • Faelen M, Resibois A, Toussaint A (1978) Mini-Mu: an insertion element derived from temperate phage Mu-1. Cold Spring Harbor Symp Quant Biol 43:1169–1177

    Google Scholar 

  • Faelen M, Toussaint A (1980) Temperate phage D108 induces chromosomal rearrangements. J Bacteriol 143:1029–1030

    Google Scholar 

  • Fry BA (1959) Conditions for the infection of E. coli with lambda phage and for establishment of lysogeny. J Gen Microbiol 21:676–689

    Google Scholar 

  • Gill GS, Hull RC, Curtiss III, R (1981) Mutator bacteriophage D108 and its DNA an electron microscopic characterization. J Virol 37:420–430

    Google Scholar 

  • Giphart-Gassler M, Reeve J, van de Putte P (1981) Polypeptides encoded by the early region of bacteriophage Mu synthetised in Minicells of E. coli. J Mol Biol 145:165–191

    Google Scholar 

  • Glansdorff N (1965) Topography of cotransducible arginine mutations in E. coli K12. Genetics 51:167–179

    Google Scholar 

  • Hattman S (1979) Unusual modification of bacteriophage Mu DNA. J Virol 32:468–475

    Google Scholar 

  • Hattman S (1980) Specificity of the bacteriophage Mu mom-controlled DNA modification. J Virol 34:277–279

    Google Scholar 

  • Howe MM (1973) Prophage deletion mapping of bacteriophage Mu-1. Virology 54:93–101

    Google Scholar 

  • Howe MM, Schumm JW, Taylor AL (1979) The S and U genes of bacteriophage Mu are located in the invertible G segment of Mu DNA. Virology 92:108–124

    Google Scholar 

  • Hsu MT, Davidson N (1974) Electron microscope heteroduplex study of the heterogeneity of Mu phage and prophage DNA. Virology 58:229–239

    Google Scholar 

  • Hull RC, Gill GS, Curtis III, R (1978) Genetic characterization of Mu-like bacteriophage D108. J Virol 27:513–518

    Google Scholar 

  • Kahmann R, Kamp D, Zipser D (1977) Mapping of restriction sites in Mu DNA. In: Bukhari AI, Shapiro JA, Adhya S (eds) DNA insertion elements, plasmids and episomes. Cold Spring Harbor Laboratory, NY, pp 335–339

    Google Scholar 

  • Khatoon H, Bukhari AI (1978) Bacteriophage Mu induced modification of DNA is dependent upon a host function. J Bacteriol 136:423–428

    Google Scholar 

  • Leach D, Symonds N (1979) The isolation and characterization of a plaque forming derivative of bacteriophage Mu carrying a fragment of Tn3 conferring ampicillin resistance. Mol Gen Genet 172:179–184

    Google Scholar 

  • Low KB (1972) Escherichia coli K12 F-prime factors, old and new. Bacteriol Rev 36:587–607

    Google Scholar 

  • Magazin M, Reeve JN, Maynard-Smith S, Symonds N (1978) Bacteriophage Mu encoded polypeptides synthesized in infected cells. FEMS Microbiol Lett 4:5–9

    Google Scholar 

  • Marinus M, Morris N (1975) Pleiotropic effects of a DNA adenine methylation mutation (dam3) in E. coli K12. Mutat Res 28:15–26

    Google Scholar 

  • Maxam A, Gilbert W (1977) A new method for sequencing DNA. Proc Natl Acad Sci/USA 74:550–564

    Google Scholar 

  • Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, NY

    Google Scholar 

  • Mise K (1971) Isolation and characterization of a new generalized transducing bacteriophage different from P1 in Escherichia coli. J Virol 7:168–175

    Google Scholar 

  • Murray K, Murray N (1975) Phage lambda receptor chromosomes for DNA fragments made with restriction endonuclease III from Haemophilus influenzae and restriction endonuclease I of E. coli. J Mol Biol 98:551–564

    Google Scholar 

  • Rambach A, Brachet P (1971) Selection de mutants du bacteriophage lambda incapables de se repliquer. CR Acad Sci (Paris) 272:149–150

    Google Scholar 

  • Resibois A, Toussaint A, Desmet L, Lefebvre N (1978) Chromosomal rearrangements induced by an IS2 insertion in phage Mu-1. Gene 4:51–68

    Google Scholar 

  • Resibois A, Toussaint A, van Gijsegem F, Faelen M (1981) Physical characterization of mini-Mu and mini-D108 derivatives. Gene 14:103–113

    Google Scholar 

  • Schumann W (1979) Cloning and biological characterization of the immunity region of Escherichia coli phage Mu. Gene 5:275–290

    Google Scholar 

  • Sharp PA, Sugden B, Sambrook J (1973) Detection of two restriction endonuclease activity in Haemophilus parainfluenzae using analytical agarose electrophoresis — Biochemistry 12:3055–3063

    Google Scholar 

  • Taylor AL (1963) Bacteriophage-induced mutation in Escherichia coli. Proc Natl Acad Sci USA 50:1043–1051

    Google Scholar 

  • Toussaint A (1976) The DNA modification function of temperate phage Mu-1. Virology 70:17–27

    Google Scholar 

  • Toussaint A (1977) The modification function of bacteriophage Mu-1 requires both a bacterial and a phage function. J Virol 23:825–826

    Google Scholar 

  • Toussaint A, Lefebvre N, Scott J, Cowan JA, De Bruijn F, Bukhari AI (1978) Relationships between temperate phages Mu and P1. Virology 89:146–161

    Google Scholar 

  • Toussaint A, Desmet L, Faelen M (1980) Mapping of the modification function of temperate phage Mu-1. Mol Gen Genet 177:351–353

    Google Scholar 

  • Toussaint A, Faelen M, Resibois A (1981) Chromosomal rearrangements induced by mini-Mu and mini-D108: mini review and new data. Gene 14:115–119

    Google Scholar 

  • van Vliet F, Couturier M, Desmet L, Faelen M, Toussaint A (1978) Virulent mutants of temperate phage Mu-1. Mol Gen Genet 160:195–202

    Google Scholar 

  • van de Putte P, Cramer S, Giphart-Gassler M (1980) Invertible DNA determines host specificity of bacteriophage Mu. Nature 286:218–222

    Google Scholar 

  • Weigle J, Meselson M, Paigen K (1959) Density alterations associated with transducing ability in the bacteriophage Lambda. J Mol Biol 1:379–386

    Google Scholar 

  • Wijffelman C, Gassler M, Stevens WF, van de Putte P (1974) On the control of transcription of bacteriophage Mu. Mol Gen Genet 131:85–96

    Google Scholar 

  • Zipser D, Moses Ph, Kahmann R, Kamp D (1977) The molecular cloning of the immunity gene of phage Mu. Gene 2:263–271

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratoire de Génétique, Faculté des Sciences, Université libre de Bruxelles, B-1640, Rhode-St-Genèse, Belgium

    Ariane Toussaint, Michel Faelen & Lucie Desmet

  2. Biogène, Carouge, Switzerland

    Bernard Allet

Authors
  1. Ariane Toussaint
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Michel Faelen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lucie Desmet
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Bernard Allet
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by W. Arber

Rights and permissions

Reprints and permissions

About this article

Cite this article

Toussaint, A., Faelen, M., Desmet, L. et al. The products of gene A of the related phages Mu and D108 differ in their specificities. Mol Gen Genet 190, 70–79 (1983). https://doi.org/10.1007/BF00330326

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00330326

Keywords

Search

Navigation