The genes for the alpha and proton-translocating subunits of wheat chloroplast ATP synthase are close together on the same strand of chloroplast DNA

Summary

The gene for the alpha subunit of chloroplast ATP synthase has been located in wheat chloroplast DNA using hybridisation to a fragment of spinach chloroplast DNA containing the alpha subunit gene, and in vitro coupled transcription-translation of cloned fragments of wheat chloroplast DNA. The gene is more than 20 kbp from the genes for the beta and epsilon subunits, but less than 2 kbp from the gene for the proton-translocating subunit (subunit III of CF0). The genes for the alpha and proton-translocating subunits are transcribed from the same DNA strand. The position of the alpha subunit gene is different from the position reported for spinach and Osmunda, probably due to an inversion of 20 kbp in wheat chloroplast DNA.

This is a preview of subscription content, access via your institution.

References

  1. Bouthyette P-Y, Jagendorf AT (1978) The site of synthesis of pea chloroplast coupling factor 1. Plant Cell Physiol 19:1169–1174

    Google Scholar 

  2. Bowman CM, Dyer TA (1982) Purification and analysis of DNA from wheat chloroplasts isolated in nonaqueous media. Anal Biochem 122:108–118

    Google Scholar 

  3. Bowman CM, Koller B, Delius H, Dyer TA (1981) A physical map of wheat chloroplast DNA showing the location of the structural genes for the ribosomal RNAs and the large subunit of ribulose 1,5-bisphosphate carboxylase. Mol Gen Genet 183:93–101

    Google Scholar 

  4. Clewell DB, Helinski DR (1969) Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an open circular DNA form. Proc Natl Acad Sci USA 62:1159–1166

    Google Scholar 

  5. Doherty A, Gray JC (1980) Synthesis of a dicyclohexylcarbodiimide-binding proteolipid by isolated pea chloroplasts. Eur J Biochem 108:131–136

    Google Scholar 

  6. Downie JA, Senior AE, Gibson F, Cox GB (1979) A fifth gene (unc E) in the operon concerned with oxidative phosphorylation in Escherichia coli. J Bacteriol 137:711–718

    Google Scholar 

  7. Dretzen G, Bellard M, Sassone-Corsi P, Chambon P (1981) A reliable method for the recovery of DNA fragments from agarose and acrylamide gels. Anal Biochem 112:295–298

    Google Scholar 

  8. Gibson F, Downie JA, Cox GB, Radik J (1978) Mu-induced polarity in the unc operon of Escherichia coli. J Bacteriol 134:728–736

    Google Scholar 

  9. Howe CJ, Bowman CM, Dyer TA, Gray JC (1982a) Localization of wheat chloroplast genes for the beta and epsilon subunits of ATP synthase. Mol Gen Genet 186:525–530

    Google Scholar 

  10. Howe CJ, Auffret AD, Doherty A, Bowman CM, Dyer TA, Gray JC (1982b) Location and nucleotide sequence of the gene for the proton-translocating subunit of wheat chloroplast ATP synthase. Proc Natl Acad Sci USA 79:6903–6907

    Google Scholar 

  11. Krebbers ET, Larrinua IM, McIntosh L, Bogorad L (1982) The maize chloroplast genes for the beta and epsilon subunits of the photosynthetic coupling factor CF1 are fused. Nucleic Acids Res 10:4985–5002

    Google Scholar 

  12. Mendiola-Morgenthaler LR, Morgenthaler J-J, Price CA (1976) Synthesis of coupling factor CF1 protein by isolated spinach chloroplasts. FEBS Lett 62:96–100

    Google Scholar 

  13. Nechushtai R, Nelson N (1981) Purification properties and biogenesis of Chlamydomonas reinhardii photosystem I reaction center. J Biol Chem 256:11624–11628

    Google Scholar 

  14. Nechushtai R, Nelson N, Mattoo AK, Edelman M (1981) Site of synthesis of subunits to photosystem I reaction center and the proton-ATPase in Spirodela. FEBS Lett 125:115–119

    Google Scholar 

  15. Nelson N, Nelson H, Schatz G (1980) Biosynthesis and assembly of the proton-translocating adenosine triphosphatase complex from chloroplasts. Proc Natl Acad Sci USA 77:1361–1364

    Google Scholar 

  16. Orozco EM, Hallick RB (1982) Euglena gracilis chloroplast transfer RNA transcription units. J Biol Chem 257:3265–3275

    Google Scholar 

  17. Palmer JD, Thompson WF (1982) Chloroplast DNA rearrangements are more frequent when a large inverted repeat sequence is lost. Cell 29:537–550

    Google Scholar 

  18. Palmer JD, Stein DB (1982) Chloroplast DNA from the fern Osmunda cinnamomea: Physical organization, gene localization and comparison to angiosperm chloroplast DNA. Curr Genet 5:165–170

    Google Scholar 

  19. van den Boogaart P, Samallo J, Agsteribbe E (1982) Similar genes for a mitochondrial ATPase subunit in the nuclear and mitochondrial genomes of Neurospora crassa. Nature 298:187–189

    Google Scholar 

  20. Westhoff P, Nelson N, Bünemann H, Herrmann RG (1981) Localization of genes for coupling factor subunits on the spinach plastid chromosome. Curr Genet 4:109–120

    Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to Christopher J. Howe.

Additional information

Communicated by H. Saedler

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Howe, C.J., Bowman, C.M., Dyer, T.A. et al. The genes for the alpha and proton-translocating subunits of wheat chloroplast ATP synthase are close together on the same strand of chloroplast DNA. Mol Gen Genet 190, 51–55 (1983). https://doi.org/10.1007/BF00330323

Download citation

Keywords

  • Subunit Gene
  • Alpha Subunit
  • Spinach Chloroplast
  • Wheat Chloroplast
  • Epsilon Subunit