Summary
Through cloning and deletion experiments on ColE1 hybrids the rpoB gene (Rifr) was located on a physical restriction map; RNA polymerase binding studies showed no binding site between rpoB and the adjacent L7/L12 ribosomal protein gene, but showed a strong binding site within the structural gene. The genetic data and RNA polymerase binding studies lead to the conclusion that rplL and rpoB are dependent upon a common promoter.
References
Burgess, R.R.: Separation and characterisation of the subunits of ribonucleic acid polymerase. J. Biol. Chem. 244, 6168–6176 (1969)
Collins, J., Fiil, N.P., Jørgensen, P., Friesen, J.O.: Gene cloning of Escherichia coli chromosomal genes important in the regulation of ribosomal RNA synthesis. In: Control of ribosome synthesis (N.O. Kjeldgaard and O. Maaløe, eds.), Alfred Benzon Symposium IX, pp. 356–369. Copenhagen: Munksgaard 1976
Collins, J., Hohn, B.: Cosmids: a type of plasmid gene-cloning vector that is packageable in vitro in bacteriophage lambda heads. Proc. Natl. Acad. Sci. U.S.A. 75, 4242–4246 (1978)
Collins, J., Johnsen, M., Jørgensen, P., Valentin-Hansen, P., Karlström, H.O., Gautier, F., Lindenmaier, W., Mayer, H., Sjöberg, B.M.: Expression of plasmid genes; ColE1 and derivatives. In: Microbiology - 1978 (Davies, J. and Novick, R., eds.), pp. 150–153. Washington: American Soc. Microbiol. (Wash.) 1978
Dougan, G., Sherratt, D.J.: The transposon Tnl as a probe for studying ColEl structure and function. Mol Gen. Genet. 151, 151–160 (1977)
Inselburg, J.: Studies of Colicin E1 plasmid functions by analysis of deletions and TnA insertions of the plasmid. J. Bacteriol. 132, 332–340 (1977)
Kirschbaum, J.L., Konrad, E.B.: Isolation of a specialised lambda transducing bacteriophage carrying the Beta subunit gene for Escherichia coli Ribonucleic acid polymerase. J. Bacteriol. 116, 517–526 (1973)
Lindahl, L., Nomura, M.: Analysis of organisation of Escherichia coli ribosome and RNA polymerase genes using biochemical methods. In: Control of ribosome synthesis (N.O. Kjeldgaard and O. Maaløe, eds.), Alfred Benzon Symposium IX, pp. 356–369 Copenhagen: Munksgaard 1976
Linn, T., Scaife, J.: Identification of a single promoter in E. coli for rplJ, rplL and rpoB,C. Nature 276, 33–37 (1978)
Seeburg, P.H., Nüsslein, C., Schaller, H.: Interaction of RNA polymerase with promoters from Bacteriophage fd. Eur. J. Biochem. 74, 107–113 (1977)
Widera, G., Gautier, F., Lindenmaier, W., Collins, J.: The expression of tetracycline resistance after insertion of foreign DNA fragments between the EcoRI and HindIII sites of the plasmid cloning vector pBR322. Mol. Gen. Genet. 163, 301–305 (1978)
Yamamoto, M., Nomura, M.: Contranscription of genes for RNA polymerase subunits and with genes for ribosomal protein in Escherichia coli. Proc. Natl Acad. Sci. U.S.A. 75, 3891–3895 (1978)
Yang, H.-L., Zubay, G.: Expression of the cel gene in ColEl and certain hybrid plasmids derived from EcoRI-treated ColE1. In: Microbiology - 1978 (Schlessinger, D., ed.), pp. 154–155. Washington: American Soc. Microbiol. (Wash.) 1978
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Communicated by E. Bautz
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Collins, J. Deletions, insertions and rearrangments affecting rpoB gene expression. Molec. gen. Genet. 173, 217–220 (1979). https://doi.org/10.1007/BF00330315
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DOI: https://doi.org/10.1007/BF00330315