Summary
Plasmid ColIb (61.5 Mdal) was digested with restriction enzymes EcoRI and HindIII. The DNA digestion products were separated by electrophoresis on 1.2% agarose gels. There were identified 22 fragments of ColIb DNA generated by the endonuclease EcoRI and 21 fragments produced by HindIII. Molecular weights of the fragments were estimated. The total molecular weight of the fragments generated by EcoRI was 61.42 Mdal and for HindIII fragments 62.79 Mdal.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Andreoli, P.M., Overbeeke, N., Veltkamp, E., Embden, J.D.A. van, Nijkamp, H.J.J.: Genetic map of the bacteriocinogenic plasmid CloDF13 derived by insertion of the transposon Tn901. Mol. Gen. Genet. 160, 1–11 (1978)
Clewell, D.B., Helinski, D.R.: Supercoiled circular DNA-protein complex in Escherichia coli: Purification and induced conversion to an open circular DNA form. Proc. Natl. Acad. Sci. U.S.A. 62, 1159–1166 (1969)
Clewell, D.B., Helinski, D.R.: Existence of the colicinogenic factorsex factor ColIb-P9 as a supercoiled circular DNA-protein relaxation complex. Biochem. Biophys. Res. Commun. 41, 150–156 (1970)
Clowes, R.: Molecular nature of bacterial plasmids. Bacteriol. Rev. 36, 361–405 (1972)
Helling, R.B., Goodman, H.M., Boyer, H.W.: Analysis of endonuclease R-EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis. J. Virol. 14, 1235–1244 (1974)
Humphreys, G.O., Willshaw, G.A., Anderson, E.S.: A simple method for the preparation of large quantities of pure plasmid DNA. Biochim. Biophys. Acta 383, 457–463 (1975)
Konisky, J., Richards, F.M.: Characterization of colicin Ia and colicin Ib. J. Biol. Chem. 245, 2972–2978 (1970)
Kozinski, A.W., Szybalski, W.: Dispersive transfer of the parental DNA molecule to the progeny of phage ϕX-174 Virology 9, 260–274 (1959)
Kushner, S.R., Nagaishi, H., Templin, A., Clark, A.J.: Genetic recombination in Escherichia coli: the role of exonuclease I. Proc. Natl. Acad. Sci. U.S.A. 68, 824–827 (1971)
Meyer, R., Figurski, D., Helinski, D.R.: Physical and Genetic studies with restriction endonucleases on the broad host-range plasmid RK2. Mol. Gen. Genet. 152, 129–135 (1977)
Modrich, P., Zabel, D.: EcoRI endonuclease. Physical and catalytic properties of the homogenous enzyme. J. Biol. Chem. 251, 5866–5874 (1976)
Oka, A., Takanami, M.: Cleavage map of plasmid ColE1. Nature 264, 193–196 (1976)
Old, R., Murray, K., Roizes, G.: Recognition sequence of restriction endonuclease III from Haemophilus influenzae. J. Mol. Biol. 92, 331–339 (1975)
Tanaka, N., Cramer, J.H., Rownd, H.: EcoRI restriction endonuclease map of the composite R plasmid NR1. J. Bacteriol. 127, 619–636 (1976)
Tanaka, N., Weisblum, B.: Construction of colicin E1-R factor composite plasmid in vitro, means for amplification of deoxyribonucleic acid. J. Bacteriol. 125, 354–362 (1975)
Thomas, M., Davis, R.W.: Studies on the cleavage of bacteriophage lambda DNA with EcoRI restriction endonuclease. J. Mol. Biol. 91, 315–328 (1975)
Timmis, K.N., Cabello, F., Cohen, S.N.: Cloning and characterization of EcoRI and HindIII restriction endonuclease — generated fragments of antibiotic resistance plasmids R6-5 and R6. Mol. Gen. Genet. 162, 121–137 (1978)
Author information
Authors and Affiliations
Additional information
Communicated by F. Kaudewitz
Rights and permissions
About this article
Cite this article
Skorupska, A., Buraczyńska, M. & Lorkiewicz, Z. Restriction enzyme analysis of the Plasmid ColIb DNA. Molec. gen. Genet. 173, 197–201 (1979). https://doi.org/10.1007/BF00330311
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00330311