Summary
A gene library of Agrobacterium tumefaciens C58 has been constructed in the plasmid vector pACYC184. A recombinant plasmid was isolated from the library by interspecific complementation in E. coli, which contained the A. tumefaciens recA gene. Heterologous Southern blotting and DNA sequence analysis have demonstrated the existence of considerable homology between the recA genes of A. tumefaciens, E. coli and R. meliloti.
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Abbreviations
- MMS:
-
methyl methanesulfonate
- UV:
-
ultraviolet light
- bp:
-
base pairs
- kbp:
-
kilo base pairs
- dATP:
-
deoxyadenosine 5′-triphosphate
- dNTP:
-
deoxynucleoside triphosphate
- Ap :
-
ampicillin
- Cm :
-
chloramphenicol
- Km :
-
kanamycin
- Tet :
-
tetracycline
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Communicated by H. Böhme
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Miles, C.A., Mountain, A. & Sastry, G.R.K. Cloning and characterisation of the recA gene of Agrobacterium tumefaciens C58. Molec Gen Genet 204, 161–165 (1986). https://doi.org/10.1007/BF00330204
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DOI: https://doi.org/10.1007/BF00330204