Summary
Termination of transcription at bacteriophage λ terminators as well as at the Escherichia coli trp a attenuator was examined in the conditionally lethal mutant (nusAts11) defective in the NusA protein of E. coli. Experiments using terminator-assay λ vectors revealed that the efficiency of termination at both ρ-dependent (λt L1) and ρ-independent (λt L2 and trp a) terminators decreases in the mutant. The mutation does not block λ phage growth at either permissive or nonpermissive temperatures, nor does it affect the λ Q protein antitermination activity at the t 6S terminator. These results indicate that NusA is required for transcription termination, and that λ N and Q-mediated antitermination may not require the NusA protein function in the nusAts11 mutant.
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References
Appleyard RK (1954) Segregation of lambda lysogenicity during bacterial recombination in E. coli K12. Genetics 39:429–439
Bachmann BJ (1983) Linkage map of Escherichia coli K-12, Edition 7. Microbiol Rev 47:180–230
Campbell A (1961) Sensitive mutants of bacteriophage λ. Virology 14:22–32
Court D, Green L, Echols H (1975) Positive and negative regulation by the cII and cIII gene products of bacteriophage λ. Virology 63:484–491
Das A, Gottesman ME, Wardwell J, Trisler P, Gottesman S (1983) A mutation in the Escherichia coli rho gene that inhibits the N protein activity of phage λ. Proc Natl Acad Sci USA 80:5530–5534
Douglas FW, Gottesman ME (1981) The nus mutations affect transcription termination in Escherichia coli. Nature (London) 292:212–215
Farnham PJ, Greenblatt J, Platt T (1982) Effects of NusA protein on transcription termination in the tryptophan operon of Escherichia coli. Cell 29:945–951
Friedman DI (1971) A bacterial mutant affecting lambda development. In: Hershey AD (ed) The bacteriophage lambda. Cold Spring Harbor Laboratory. New York, pp 733–738
Friedman DI, Baron LS (1974) Genetic characterization of a bacterial locus involved in the activity of the N function of phage λ. Virology 58:141–148
Friedman DI, Gottesman M (1983) The lytic mode of development. In: Hendrix RW, Roberts JW, Stahl FW, Weisberg RA (eds) Lambda II. Cold Spring Harbor Laboratory. New York, pp 21–51
Grayhack EJ, Roberts JW (1982) The phage λ Q gene product: activity of a transcription antitermination in vitro. Cell 30:637–648
Greenblatt J, McLimont M, Hanly S (1981) Termination of transcription by nusA gene protein of Escherichia coli. Nature (London) 292:215–220
Kassavetis GA, Chamberlin MJ (1981) Pausing and termination of transcription within the early region of bacteriophage T7 DNA in vitro. J Biol Chem 256:2777–2786
Kingston RE, Chamberlin MJ (1981) Pausing and attenuation of in vitro transcription in the rrnB operon of E. coli. Cell 27:523–531
Kung H-F, Spears C, Weissbach H (1975) Purification and properties of a soluble factor required for the deoxyribonucleic acid-directed in vitro synthesis of β-galactosidase. J Biol Chem 250:1556–1562
McMacken R, Mantei N, Butler B, Joyner A, Echols H (1970) Effect of mutations in the cII and cIII genes of bacteriophage λ on macromolecular synthesis in infected cells. J Mol Biol 49:639–655
Miller J (1972) In: Experiments in Molecular Biology. Cold Spring Harbor Laboratory, New York
Nakamura Y, Tsugawa A (1985) Role of Escherichia coli NusA protein in transcription termination: ts mutant and RNA recognition. In: Calendar R, Gold L (eds) Sequence specificity in transcription and translation. UCLA Symposia on Molecular and Cellular Biology, New Series, vol 30. Alan R Liss, Inc., New York, pp 185–196
Nakamura Y, Uchida H (1983) Isolation of conditionally lethal amber mutations affecting synthesis of the nusA protein of Escherichia coli. Mol Gen Genet 190:196–203
Olson ER, Flamm EL, Friedman DI (1982) Analysis of nutR: a region of phage lambda required for antitermination of transcription. Cell 31:61–70
Schmidt MC, Chamberlin MJ (1984) Binding of rho factor to Escherichia coli RNA polymerase mediated by nusA protein. J Biol Chem 259:15000–15002
Tsurushita N, Hirano M, Shigesada K, Imai M (1984) Isolation and characterization of rho mutants of Escherichia coli with increased transcription termination activities. Mol Gen Genet 196:458–464
Zarucki-Schulz T, Jerez C, Goldberg G, Kung H-F, Huang K-H, Brot N, Weissbach H (1979) DNA-directed in vitro synthesis of proteins involved in bacterial transcription and translation. Proc Natl Acad Sci USA 76:6115–6119
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Nakamura, Y., Mizusawa, S., Tsugawa, A. et al. Conditionally lethal nusAts mutation of Escherichia coli reduces transcription termination but does not affect antitermination of bacteriophage lambda. Molec Gen Genet 204, 24–28 (1986). https://doi.org/10.1007/BF00330182
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DOI: https://doi.org/10.1007/BF00330182